Rv2573 2-dehydropantoate 2-reductase (EC 1.1.1.169)

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv2573 2-dehydropantoate 2-reductase (EC 1.1.1.169) CDS 2897956 2898783 + 828 275 FALSE

Rv2573 (2-dehydropantoate 2-reductase (EC 1.1.1.169)) is predicted to be co-regulated in modules bicluster_0185 with residual 0.52 and bicluster_0552 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,500.00 and 29,000.00 for bicluster_0185 and 0.00 and 0.00 for bicluster_0552 respectively.

These modules are enriched for following go terms: IMP biosynthetic process, 'de novo' IMP biosynthetic process, IMP metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Putative 2-dehydropantoate 2-reductase 2-dehydropantoate 2-reductase
Operon # Operon
1690 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

2-dehydropantoate 2-reductase Pantothenate and CoA biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pantothenate and CoA biosynthesis

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
161352464 NP_217089.2 Run
GO:0008677

2-dehydropantoate 2-reductase activity

2-dehydropantoate 2-reductase activity

Details: 
Catalysis of the reaction: (R)-pantoate + NADP(+) = 2-dehydropantoate + H(+) + NADPH.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.850000 1.32

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: