Rv2727c tRNA delta(2)-isopentenylpyrophosphate transferase (EC 2.5.1.8)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2727c miaA tRNA delta(2)-isopentenylpyrophosphate transferase (EC 2.5.1.8) CDS 3039825 3040769 - 945 314 FALSE

Rv2727c (tRNA delta(2)-isopentenylpyrophosphate transferase (EC 2.5.1.8)) is predicted to be co-regulated in modules bicluster_0398 with residual 0.53 and bicluster_0468 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.30 and 23.00 for bicluster_0398 and 14.00 and 16.00 for bicluster_0468 respectively.

These modules are enriched for following go terms: ligase activity, forming carbon-nitrogen... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
tRNA delta[2]-isopentenylpyrophosphate transferase tRNA delta(2)-isopentenylpyrophosphate transferase
Operon # Operon
1793 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

tRNA dimethylallyltransferase Zeatin biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609864 NP_217243.1 Run
GO:0052381

tRNA dimethylallyltransferase activity

tRNA dimethylallyltransferase activity

Details: 
Catalysis of the reaction: dimethylallyl diphosphate + tRNA = diphosphate + tRNA containing 6-dimethylallyladenosine.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: