Rv2737c RecA protein @ intein-containing

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2737c recA RecA protein @ intein-containing CDS 3049052 3051424 - 2 373 790 FALSE

Rv2737c (RecA protein @ intein-containing) is predicted to be co-regulated in modules bicluster_0031 with residual 0.69 and bicluster_0250 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0031 and 91.00 and 3,000.00 for bicluster_0250 respectively.

These modules are enriched for following go terms: damaged DNA binding, cysteine-type endopeptidase activity, cysteine-type peptidase activity single-organism process, nucleic acid metabolic process, hydrolase activity, endodeoxyribonuclease activity, serine-type endopeptidase activity, nucleoside-triphosphatase activity, pyrophosphatase activity, hydrolase activity, acting on acid anhyd..., hydrolase activity, acting on acid anhyd..., deoxyribonuclease activity, ATPase activity, coupled, DNA-dependent ATPase activity, DNA helicase activity, serine-type peptidase activity, serine hydrolase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:17
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15047 MT2806 1246
Product (LegacyBRC) Product (RefSeq)
Protein recA DNA recombination protein RecA
Operon # Operon
1800 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Homologous recombination

22
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609874 NP_217253.1 Run
GO:0000150

recombinase activity

recombinase activity

Details: 
Catalysis of the identification and base-pairing of homologous sequences between single-stranded DNA and double-stranded DNA.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0000725

recombinational repair

recombinational repair

Details: 
A DNA repair process that involves the exchange, reciprocal or nonreciprocal, of genetic material between the broken DNA molecule and a homologous region of DNA.
GO Category: 
biological_process
1
Total items in this category:  
GO:0003697

single-stranded DNA binding

single-stranded DNA binding

Details: 
Interacting selectively and non-covalently with single-stranded DNA.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004520

endodeoxyribonuclease activity

endodeoxyribonuclease activity

Details: 
Catalysis of the hydrolysis of ester linkages within deoxyribonucleic acid by creating internal breaks.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0006974

response to DNA damage stimulus

response to DNA damage stimulus

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
GO Category: 
biological_process
8
Total items in this category:  
GO:0008094

DNA-dependent ATPase activity

DNA-dependent ATPase activity

Details: 
Catalysis of the reaction: ATP + H2O = ADP + phosphate; this reaction requires the presence of single- or double-stranded DNA, and it drives another reaction.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0009650

UV protection

UV protection

Details: 
Any process in which an organism or cell protects itself from ultraviolet radiation (UV), which may also result in resistance to repeated exposure to UV.
GO Category: 
biological_process
2
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.070000 0.95

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: