Rv2761c Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2761c hsdS Type I restriction-modification system, specificity subunit S (EC 3.1.21.3) CDS 3071546 3072640 - 1 095 364 FALSE

Rv2761c (Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)) is predicted to be co-regulated in modules bicluster_0081 with residual 0.55 and bicluster_0242 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.53 and 19,000.00 for bicluster_0081 and 0.00 and 0.00 for bicluster_0242 respectively.

These modules are enriched for following go terms: DNA modification.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:17
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18110 MT2831 1426
Product (LegacyBRC) Product (RefSeq)
POSSIBLE TYPE I RESTRICTION_MODIFICATION SYSTEM SPECIFICITY DETERMINANT HSDS [S PROTEIN] type I restriction/modification system specificity determinant HsdS
Operon # Operon
1814 - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609898 NP_217277.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.130000 1.03

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: