Rv2888c Putative amidase amiC (EC


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2888c amiC Putative amidase amiC (EC CDS 3196864 3198285 - 1 422 473 FALSE

Rv2888c (Putative amidase amiC (EC is predicted to be co-regulated in modules bicluster_0327 with residual 0.42 and bicluster_0462 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 440.00 and 980.00 for bicluster_0327 and 2,900.00 and 12,000.00 for bicluster_0462 respectively.

These modules are enriched for following go terms: monocarboxylic acid binding, protein tyrosine kinase activity, sulfur compound binding, protein kinase activity, amide binding .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18274 MT2956 1909
Product (LegacyBRC) Product (RefSeq)
Putative amidase amiC amidase
Operon # Operon
1888 - -
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Arginine and proline metabolism

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Phenylalanine metabolism

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Tryptophan metabolism

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Cyanoamino acid metabolism

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Aminobenzoate degradation

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Styrene degradation

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Microbial metabolism in diverse environments

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15610025 NP_217404.1 Run

plasma membrane

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.98

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: