Rv2911 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2911 dacB2 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) CDS 3218339 3219214 + 876 291 FALSE

Rv2911 (D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)) is predicted to be co-regulated in modules bicluster_0065 with residual 0.72 and bicluster_0208 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 7.10 and 1,500.00 for bicluster_0065 and 2,200.00 and 230.00 for bicluster_0208 respectively.

These modules are enriched for following go terms: UDP-glycosyltransferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15724 MT2979 2313
Product (LegacyBRC) Product (RefSeq)
PROBABLE D-ALANYL-D-ALANINE CARBOXYPEPTIDASE DACB2 [PENICILLIN-BINDING PROTEIN] [DD-PEPTIDASE] [DD-CARBOXYPEPTIDASE] [PBP] [DD-TRANSPEPTIDASE] [SERINE-TYPE D-ALA-D-ALA CARBOXYPEPTIDASE] [D-AMINO ACID HYDROLASE] D-alanyl-D-alanine carboxypeptidase
Operon # Operon
1901
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Serine-type D-Ala-D-Ala carboxypeptidase Peptidoglycan biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Peptidoglycan biosynthesis

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117035 YP_177914.1 Run
GO:0009002

serine-type D-Ala-D-Ala carboxypeptidase activity

serine-type D-Ala-D-Ala carboxypeptidase activity

Details: 
Catalysis of the reaction: (Ac)2-L-Lys-D-alanyl-D-alanine + H2O = (Ac)2-L-Lys-D-alanine + D-alanine.
GO Category: 
molecular_function
3
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.090000 0.56

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: