Rv2973c ATP-dependent DNA helicase RecG (EC 3.6.1.-)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2973c recG ATP-dependent DNA helicase RecG (EC 3.6.1.-) CDS 3327733 3329946 - 2 214 737 FALSE

Rv2973c (ATP-dependent DNA helicase RecG (EC 3.6.1.-)) is predicted to be co-regulated in modules bicluster_0189 with residual 0.44 and bicluster_0589 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 13,000.00 and 13,000.00 for bicluster_0189 and 0.72 and 69.00 for bicluster_0589 respectively.

These modules are enriched for following go terms: glycerol metabolic process, alditol metabolic process, polyol metabolic process, alcohol metabolic process, organic hydroxy compound metabolic proce..., aspartic-type endopeptidase activity, aspartic-type peptidase activity, ATP-dependent DNA helicase activity, DNA-dependent ATPase activity kinase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:41
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14735 MT3051 491
Product (LegacyBRC) Product (RefSeq)
ATP-dependent DNA helicase recG ATP-dependent DNA helicase RecG
Operon # Operon
1941 - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Homologous recombination

22
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610110 NP_217489.1 Run
GO:0003676

nucleic acid binding

nucleic acid binding

Details: 
Interacting selectively and non-covalently with any nucleic acid.
GO Category: 
molecular_function
13
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0008026

ATP-dependent helicase activity

ATP-dependent helicase activity

Details: 
Catalysis of the reaction: ATP + H2O = ADP + phosphate, to drive the unwinding of a DNA or RNA helix.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0004386

helicase activity

helicase activity

Details: 
Catalysis of the reaction: NTP + H2O = NDP + phosphate, to drive the unwinding of a DNA or RNA helix.
GO Category: 
molecular_function
6
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.200000 2.68

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: