Rv3276c Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3276c purK Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC CDS 3658635 3659924 - 1 290 429 FALSE

Rv3276c (Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC is predicted to be co-regulated in modules bicluster_0554 with residual 0.55 and bicluster_0581 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 8.40 and 31.00 for bicluster_0554 and 0.01 and 120.00 for bicluster_0581 respectively.

These modules are enriched for following go terms: cellular amide metabolic process, amide biosynthetic process .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.377 9 3659915 54 3659861 3659924
Product (LegacyBRC) Product (RefSeq)
Phosphoribosylaminoimidazole carboxylase ATPase subunit phosphoribosylaminoimidazole carboxylase ATPase subunit
Operon # Operon
2142 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Phosphoribosylaminoimidazole carboxylase Purine metabolism
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Purine metabolism

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Metabolic pathways

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Biosynthesis of secondary metabolites

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15610412 NP_217793.1 Run

phosphoribosylaminoimidazole carboxylase activity

phosphoribosylaminoimidazole carboxylase activity

Catalysis of the reaction: 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + 2 H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2).
GO Category: 
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plasma membrane

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
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No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: