Rv3399 O-Methyltransferase involved in polyketide biosynthesis

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv3399 O-Methyltransferase involved in polyketide biosynthesis CDS 3816129 3817175 + 1 047 348 FALSE

Rv3399 (O-Methyltransferase involved in polyketide biosynthesis) is predicted to be co-regulated in modules bicluster_0140 with residual 0.62 and bicluster_0394 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0140 and 26.00 and 340.00 for bicluster_0394 respectively.

These modules are enriched for following go terms: cellular protein metabolic process, macromolecular complex, cytoplasmic part.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.719 3816255 3816129
Last update: 10/16/2017 - 15:33
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18380 MT3507 2141
Product (LegacyBRC) Product (RefSeq)
Putative S-adenosyl-L-methionine-dependent methyltransferase Rv3399_MT3507
Operon # Operon
2224
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610535 NP_217916.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.480000 0.90

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: