Rv3421c Inactive homolog of metal-dependent proteases, putative molecular chaperone

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv3421c Inactive homolog of metal-dependent proteases, putative molecular chaperone CDS 3839059 3839694 - 636 211 FALSE

Rv3421c (Inactive homolog of metal-dependent proteases, putative molecular chaperone) is predicted to be co-regulated in modules bicluster_0192 with residual 0.54 and bicluster_0460 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 4,500.00 and 12,000.00 for bicluster_0192 and 55.00 and 160.00 for bicluster_0460 respectively.

These modules are enriched for following go terms: cellular macromolecular complex assembly, cellular protein complex assembly, protein complex assembly, macromolecular complex assembly, protein complex biogenesis branched-chain amino acid metabolic proc..., translation, acetolactate synthase activity, anion transmembrane-transporting ATPase ..., structural constituent of ribosome.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.282 3839706 3839694
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv3421c_MT3530
Operon # Operon
2237 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610557 NP_217938.1 Run
GO:0008450

O-sialoglycoprotein endopeptidase activity

O-sialoglycoprotein endopeptidase activity

Details: 
OBSOLETE. Catalysis of the hydrolysis of O-sialoglycoproteins; cleaves the -Arg31-Asp32- bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0006508

proteolysis

proteolysis

Details: 
The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.
GO Category: 
biological_process
4
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.040000 0.05

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: