Rv3820c Polyketide synthase associated protein papA2

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3820c papA2 Polyketide synthase associated protein papA2 CDS 4284419 4285825 - 1 407 468 FALSE

Rv3820c (Polyketide synthase associated protein papA2) is predicted to be co-regulated in modules bicluster_0229 with residual 0.54 and bicluster_0247 with residual 0.41.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 170.00 and 860.00 for bicluster_0229 and 430.00 and 9,100.00 for bicluster_0247 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.043 4285645 4285825
Last update: 10/16/2017 - 16:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15051 MT3928 1256
Product (LegacyBRC) Product (RefSeq)
Trehalose-2-sulfate acyltransferase papA2 polyketide synthase associated protein PapA2
Operon # Operon
2498
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57117162 YP_178020.1 Run
GO:0008415

NA

NA

Details: 
NA
GO Category: 
NA
6
Total items in this category:  
GO:0046506

sulfolipid biosynthetic process

sulfolipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of sulfolipid, a compound containing a sulfonic acid residue joined by a carbon-sulfur bond to a lipid.
GO Category: 
biological_process
5
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.38

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: