Rv3853 Ribonuclease E inhibitor RraA


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3853 menG Ribonuclease E inhibitor RraA CDS 4325495 4325968 + 474 157 FALSE

Rv3853 (Ribonuclease E inhibitor RraA) is predicted to be co-regulated in modules bicluster_0281 with residual 0.48 and bicluster_0585 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 140.00 and 1,200.00 for bicluster_0281 and 0.06 and 350.00 for bicluster_0585 respectively.

These modules are enriched for following go terms: acetate-CoA ligase activity cofactor biosynthetic process, coenzyme metabolic process, cofactor metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Regulator of ribonuclease activity A ribonuclease activity regulator protein RraA
Operon # Operon
2521 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15610989 NP_218370.1 Run

protein homotrimerization

protein homotrimerization

The formation of a protein homotrimer, a macromolecular structure consisting of three noncovalently associated identical subunits.
GO Category: 
Total items in this category:  

S-adenosylmethionine-dependent methyltransferase activity

S-adenosylmethionine-dependent methyltransferase activity

Catalysis of the transfer of a methyl group from S-adenosyl-L-methionine to a substrate.
GO Category: 
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.390000 0.90

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: