Organism : Bacillus cereus ATCC14579 | Module List :
BC4614

Xaa-Pro dipeptidase (NCBI ptt file)

CircVis
Functional Annotations (6)
Function System
Xaa-Pro aminopeptidase cog/ cog
methionyl aminopeptidase activity go/ molecular_function
X-Pro dipeptidase activity go/ molecular_function
proteolysis go/ biological_process
creatine metabolic process go/ biological_process
creatinase activity go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for BC4614
(Mouseover regulator name to see its description)

BC4614 is regulated by 22 influences and regulates 0 modules.
Regulators for BC4614 (22)
Regulator Module Operator
BC0880 152 tf
BC1302 152 tf
BC1356 152 tf
BC1490 152 tf
BC1998 152 tf
BC2358 152 tf
BC4289 152 tf
BC4603 152 tf
BC4670 152 tf
BC5483 152 tf
BC0057 474 tf
BC0123 474 tf
BC0158 474 tf
BC0880 474 tf
BC1477 474 tf
BC1818 474 tf
BC3128 474 tf
BC4336 474 tf
BC4603 474 tf
BC4672 474 tf
BC5282 474 tf
BC5339 474 tf

Warning: BC4614 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
4220 7.20e-02 ccCTCC
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4221 1.40e+02 gAGTAcaaagTGAgAA
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4858 1.80e+01 gtaAaAAtgtGaaaaGaagtg
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4859 5.70e+02 TAGGGgG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for BC4614

BC4614 is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
Xaa-Pro aminopeptidase cog/ cog
methionyl aminopeptidase activity go/ molecular_function
X-Pro dipeptidase activity go/ molecular_function
proteolysis go/ biological_process
creatine metabolic process go/ biological_process
creatinase activity go/ molecular_function
Module neighborhood information for BC4614

BC4614 has total of 30 gene neighbors in modules 152, 474
Gene neighbors (30)
Gene Common Name Description Module membership
BC0072 BC0072 Cell division protein ftsH (NCBI ptt file) 110, 152
BC0512 BC0512 Glutamate-1-semialdehyde 2,1-aminomutase (NCBI ptt file) 110, 152
BC0584 BC0584 Acetyltransferase (NCBI ptt file) 152, 262
BC0667 BC0667 Bicyclomycin resistance protein (NCBI ptt file) 152, 403
BC0850 BC0850 Oxidoreductase (NCBI ptt file) 201, 474
BC1200 BC1200 Ribosomal large subunit pseudouridine synthase D (NCBI ptt file) 403, 474
BC1295 BC1295 hypothetical protein (NCBI ptt file) 201, 474
BC1371 BC1371 Protein dltB (NCBI ptt file) 201, 474
BC1498 BC1498 SSU ribosomal protein S1P (NCBI ptt file) 107, 152
BC1511 BC1511 GTP cyclohydrolase I (NCBI ptt file) 110, 152
BC2157 BC2157 hypothetical protein (NCBI ptt file) 110, 152
BC2191 BC2191 Thymidylate synthase (NCBI ptt file) 300, 474
BC2204 BC2204 Asparagine synthetase [glutamine-hydrolyzing] (NCBI ptt file) 474, 477
BC3461 BC3461 LMBE-related protein (NCBI ptt file) 152, 268
BC3682 BC3682 Transketolase (NCBI ptt file) 296, 474
BC3773 BC3773 Pyruvate synthase subunit porB (NCBI ptt file) 110, 152
BC3797 BC3797 Dihydrodipicolinate synthase (NCBI ptt file) 371, 474
BC3862 BC3862 Radical SAM family enzyme (NCBI ptt file) 367, 474
BC3895 BC3895 Isoleucyl-tRNA synthetase (NCBI ptt file) 22, 474
BC3950 BC3950 hypothetical protein (NCBI ptt file) 152, 180
BC3977 BC3977 Zn-dependent hydrolase (NCBI ptt file) 226, 474
BC4151 BC4151 hypothetical protein (NCBI ptt file) 107, 152
BC4392 BC4392 Cysteine desulfhydrase (NCBI ptt file) 367, 474
BC4397 BC4397 Aspartyl-tRNA synthetase (NCBI ptt file) 379, 474
BC4479 BC4479 ATP-dependent clp protease ATP-binding subunit clpX (NCBI ptt file) 110, 152
BC4603 BC4603 Transcriptional regulator, GntR family (NCBI ptt file) 403, 474
BC4614 BC4614 Xaa-Pro dipeptidase (NCBI ptt file) 152, 474
BC4979 BC4979 ABC transporter-associated protein (NCBI ptt file) 152, 164
BC5188 BC5188 Bacterial Peptide Chain Release Factor 2 (RF-2) (NCBI ptt file) 201, 474
BC5392 BC5392 hypothetical Membrane Spanning Protein (NCBI ptt file) 110, 152
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for BC4614
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend