Organism : Bacillus subtilis | Module List:
Module 64 Profile

GeneModule member RegulatorRegulator MotifMotif
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 64

There are 2 regulatory influences for Module 64

Regulator Table (2)
Regulator Name Type
BSU05850 tf
BSU09650 tf

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
5084 4.70e-02 aaaaAggggaG
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5085 1.20e+02 TaTATTTTaccATtAaActGTgAC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 64 is enriched for following functions.

Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 64

There are 32 genes in Module 64

Gene Member Table (32)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
BSU00990 rpmG CDS None chromosome 117346 117495 + 50S ribosomal protein L33 (RefSeq) False
BSU03450 hxlB CDS None chromosome 374162 374719 - 6-phospho-3-hexuloisomerase (PHI) (RefSeq) False
BSU03460 hxlA CDS None chromosome 374725 375357 - 3-hexulose-6-phosphate synthase (HPS) (RefSeq) False
BSU04020 yczH CDS None chromosome 454257 454814 - putative hydrolase (RefSeq) False
BSU09540 plsC CDS None chromosome 1030703 1031302 + 1-acylglycerol-phosphate (1-acyl-G3P) acyltransferase (RefSeq) False
BSU09600 crcBA CDS None chromosome 1036261 1036617 - camphor resistance protein CrcB (RefSeq) False
BSU10010 trpP CDS None chromosome 1073951 1074469 - tryptophan transporter (RefSeq) False
BSU10880 yisV CDS None chromosome 1166042 1167496 + putative PLP-dependent transcriptional regulator (RefSeq) True
BSU10990 yitH CDS None chromosome 1176670 1177518 - putative N-acetyltransferase (RefSeq) False
BSU11000 yitI CDS None chromosome 1177523 1177987 - putative N-acetyltransferase (RefSeq) False
BSU11270 yjzD CDS None chromosome 1204038 1204223 - hypothetical protein (RefSeq) False
BSU11280 yjaU CDS None chromosome 1204472 1205206 + hypothetical protein (RefSeq) False
BSU11480 yjbB CDS None chromosome 1224870 1226087 + putative exporter (RefSeq) False
BSU11510 yjbE CDS None chromosome 1227448 1228104 - putative transporter component (RefSeq) False
BSU12500 xkdA CDS None chromosome 1319881 1320477 - PBSX phage protein, putative peptidase (RefSeq) False
BSU13070 ykkA CDS None chromosome 1374565 1375092 + hypothetical protein (RefSeq) False
BSU13080 ykkB CDS None chromosome 1375082 1375600 + putative N-acetyltransferase (RefSeq) False
BSU15420 divIVA CDS None chromosome 1611824 1612318 + cell-division initiation protein (RefSeq) False
BSU18620 yoaI CDS None chromosome 2030972 2032099 + putative 4-hydroxyphenylacetate-3-hydroxylase (RefSeq) False
BSU18690 yoaP CDS None chromosome 2038819 2039574 - hypothetical protein (RefSeq) False
BSU19090 yobU CDS None chromosome 2081742 2082224 - putative effector of transcriptional regulator (RefSeq) True
BSU19100 yobV CDS None chromosome 2082278 2083219 - putative transcriptional regulator (RefSeq) True
BSU21830 ypjQ CDS None chromosome 2297180 2297713 - putative phosphatidylglycerophosphatase (RefSeq) False
BSU26230 yqaP CDS None chromosome 2690868 2691797 + conserved hypothetical protein; skin element (RefSeq) False
BSU26240 yqaO CDS None chromosome 2691871 2692077 - conserved hypothetical protein; skin element (RefSeq) False
BSU27800 yrzH CDS None chromosome 2840394 2840525 + hypothetical protein (RefSeq) False
BSU27810 yrbD CDS None chromosome 2840836 2842290 + sodium/proton-dependent alanine transporter (RefSeq) False
BSU30670 luxS CDS None chromosome 3136543 3137016 - S-ribosylhomocysteinase (RefSeq) False
BSU30680 ytjA CDS None chromosome 3137145 3137372 + hypothetical protein (RefSeq) False
BSU31580 maeN CDS None chromosome 3243814 3245160 + Na+/malate symporter (RefSeq) False
BSU35880 pgsA CDS None chromosome 3696848 3697990 - capsular polyglutamate synthetase (RefSeq) False
BSU36320 ywpG CDS None chromosome 3739651 3740034 - hypothetical protein (RefSeq) False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.