Organism : Clostridium acetobutylicum | Module List :
CAC1378 cbiT

Precorrin-6B methylase CbiT (NCBI ptt file)

CircVis
Functional Annotations (8)
Function System
Precorrin-6B methylase 2 cog/ cog
cytoplasm go/ cellular_component
protein methylation go/ biological_process
cell cycle go/ biological_process
protein methyltransferase activity go/ molecular_function
cobalamin biosynthetic process go/ biological_process
precorrin-6Y C5,15-methyltransferase (decarboxylating) activity go/ molecular_function
CbiT tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC1378
(Mouseover regulator name to see its description)

CAC1378 is regulated by 21 influences and regulates 0 modules.
Regulators for CAC1378 cbiT (21)
Regulator Module Operator
CAC0144 56 tf
CAC0393 56 tf
CAC0708 56 tf
CAC0745 56 tf
CAC0821 56 tf
CAC2071 56 tf
CAC2307 56 tf
CAC2473 56 tf
CAC2773 56 tf
CAC2842 56 tf
CAC2962 56 tf
CAC3214 56 tf
CAC0201 234 tf
CAC0459 234 tf
CAC0627 234 tf
CAC1032 234 tf
CAC1766 234 tf
CAC1800 234 tf
CAC2473 234 tf
CAC2842 234 tf
CAC3199 234 tf

Warning: CAC1378 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
6766 5.10e+01 ActatGCTCtT
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6767 5.80e+02 TA.tGgGcTTt.act
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7120 8.70e+02 gAggAggg
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7121 1.30e+04 GcGAAACTAG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC1378

CAC1378 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
Precorrin-6B methylase 2 cog/ cog
cytoplasm go/ cellular_component
protein methylation go/ biological_process
cell cycle go/ biological_process
protein methyltransferase activity go/ molecular_function
cobalamin biosynthetic process go/ biological_process
precorrin-6Y C5,15-methyltransferase (decarboxylating) activity go/ molecular_function
CbiT tigr/ tigrfam
Module neighborhood information for CAC1378

CAC1378 has total of 31 gene neighbors in modules 56, 234
Gene neighbors (31)
Gene Common Name Description Module membership
CAC0490 CAC0490 Predicted sugar kinase, N-terminal region - uncharacterized conserved protein (NCBI ptt file) 29, 56
CAC0491 CAC0491 Uncharacterized probably secreted protein, homolog of YDCC B.subtilis (NCBI ptt file) 56, 315
CAC0776 CAC0776 NCAIR mutase (PurE)-related protein (NCBI ptt file) 234, 316
CAC0822 CAC0822 ABC transporter, ATP-binding protein (NCBI ptt file) 234, 271
CAC0837 potD Spermidine/putrescine-binding periplasmic protein (NCBI ptt file) 56, 153
CAC0838 potC ABC-type spermidine/putrescine transport system, permease component II (NCBI ptt file) 56, 153
CAC0842 CAC0842 Hypothetical protein, CF-28 family (NCBI ptt file) 38, 56
CAC0998 CAC0998 Homoserine dehydrogenase (NCBI ptt file) 234, 256
CAC0999 thrC Threonine synthase (NCBI ptt file) 234, 256
CAC1091 CAC1091 Aspartyl aminopeptidase (NCBI ptt file) 159, 234
CAC1100 CAC1100 Secreted protein, similar to catalytic domain of murein transglycosylase (NCBI ptt file) 38, 56
CAC1246 pbpA Penicillin-binding protein 2 (NCBI ptt file) 56, 200
CAC1366 CAC1366 Predicted membrane protein (NCBI ptt file) 234, 357
CAC1374 cbiP Cobyric acid synthase CbiP (NCBI ptt file) 56, 234
CAC1375 cobB Cobyrinic acid a,c-diamide synthase CobB (NCBI ptt file) 56, 234
CAC1376 cbiC/cobH Precorrin isomerase, cbiC (NCBI ptt file) 55, 56
CAC1377 cbiD Cobalamin biosynthesis protein CbiD (NCBI ptt file) 56, 234
CAC1378 cbiT Precorrin-6B methylase CbiT (NCBI ptt file) 56, 234
CAC1379 cobI/cbiL Precorrin-2 methylase CobI/CbiL (NCBI ptt file) 56, 234
CAC1380 cbiF/cobM precorrin-4 methylase cbiF (NCBI ptt file) 56, 234
CAC1382 cbiH/cobJ precorrin-3 methylase (NCBI ptt file) 56, 234
CAC1383 CobU/CobP Adenosyl cobinamide kinase/adenosyl cobinamide phosphate (NCBI ptt file) 56, 234
CAC1385 cobC Alpha-ribazole-5'-phosphate phosphatase, CobC (NCBI ptt file) 56, 234
CAC1715 CAC1715 PLP-dependent aminotransferase (NCBI ptt file) 159, 234
CAC1721 rpiA Primosomal protein N', superfamily II helicase (NCBI ptt file) 172, 234
CAC2268 CAC2268 Uncharacterized protein of YOHL family (NCBI ptt file) 163, 234
CAC2282 tgt Queuine tRNA-ribosyltransferase, tgt (NCBI ptt file) 229, 234
CAC2328 CAC2328 Polysaccharide ABC transporter, ATPase component (NCBI ptt file) 234, 327
CAC2530 CAC2530 Predicted N-acetyltransferase (NCBI ptt file) 234, 274
CAC2573 CAC2573 Predicted S-adenosylmethionine-dependent methyltransferase (NCBI ptt file) 56, 234
CAC2834 CAC2834 Uncharacterized conserved protein, YHAD family (NCBI ptt file) 234, 288
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC1378
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend