Organism : Clostridium acetobutylicum | Module List :
CAC2275 apt

Adenine phosphoribosyltransferase; Apt (NCBI ptt file)

CircVis
Functional Annotations (5)
Function System
Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins cog/ cog
adenine phosphoribosyltransferase activity go/ molecular_function
adenine salvage go/ biological_process
nucleoside metabolic process go/ biological_process
apt tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC2275
(Mouseover regulator name to see its description)

CAC2275 is regulated by 18 influences and regulates 0 modules.
Regulators for CAC2275 apt (18)
Regulator Module Operator
CAC0162 276 tf
CAC0821 276 tf
CAC0876 276 tf
CAC1668 276 tf
CAC1766 276 tf
CAC1800 276 tf
CAC2546 276 tf
CAC0162 152 tf
CAC0183 152 tf
CAC0459 152 tf
CAC0465 152 tf
CAC0977 152 tf
CAC1675 152 tf
CAC1698 152 tf
CAC2236 152 tf
CAC2546 152 tf
CAC3037 152 tf
CAC3647 152 tf

Warning: CAC2275 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
6956 2.40e-02 tTtC.CC.ttc
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6957 2.40e+04 GCTCAC
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7204 1.70e-02 aGGaggaA
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7205 2.70e+01 TaAgacaGaaGAGGT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC2275

CAC2275 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins cog/ cog
adenine phosphoribosyltransferase activity go/ molecular_function
adenine salvage go/ biological_process
nucleoside metabolic process go/ biological_process
apt tigr/ tigrfam
Module neighborhood information for CAC2275

CAC2275 has total of 36 gene neighbors in modules 152, 276
Gene neighbors (36)
Gene Common Name Description Module membership
CAC0083 CAC0083 PemK family of DNA-binding proteins (NCBI ptt file) 152, 260
CAC0094 CAC0094 Ferredoxin-nitrite reductase (NCBI ptt file) 27, 276
CAC0162 CAC0162 Transcriptional regulator MarR/EmrR family (NCBI ptt file) 83, 276
CAC0268 CAC0268 ABC transporter ATP-binding protein (NCBI ptt file) 27, 276
CAC0269 CAC0269 Uncharacterized membrane protein (NCBI ptt file) 27, 276
CAC0292 CAC0292 Hypothetical protein (NCBI ptt file) 162, 276
CAC0459 CAC0459 NtrC family transcriptional regulator (PAS and AAA domains) (NCBI ptt file) 92, 152
CAC0500 CAC0500 Membrane protein containing C-terminal PDZ domain (NCBI ptt file) 152, 182
CAC0515 CAC0515 Uncharacterized conserved protein (NCBI ptt file) 25, 276
CAC0950 CAC0950 Predicted membrane protein (NCBI ptt file) 152, 233
CAC0954 CAC0954 Uncharacterized membrane protein (NCBI ptt file) 25, 276
CAC0969 CAC0969 Hypothetical protein (NCBI ptt file) 152, 182
CAC0977 asnC Transcriptional regulator, Lrp family (NCBI ptt file) 152, 268
CAC1028 CAC1028 Hydrolase of alpha/beta superfamily, possible membrane associated lipase (NCBI ptt file) 276, 356
CAC1050 nadE NH(3)-dependent NAD(+) synthetase (NCBI ptt file) 31, 276
CAC1121 CAC1121 Predicted membrane protein (NCBI ptt file) 134, 152
CAC1214 CAC1214 Xre family DNA-binding domain and TPR-repeat containing protein (NCBI ptt file) 152, 268
CAC1434 CAC1434 Alkaline phosphatase superfamily protein (NCBI ptt file) 176, 276
CAC1683 CAC1683 Predicted metal-dependent hydrolase of metallo-beta-lactamase superfamily (NCBI ptt file) 239, 276
CAC1698 CAC1698 Uncharacterized conserved protein, YTCG B.subtilis ortholog (NCBI ptt file) 130, 152
CAC1868 CAC1868 Uncharacterized secreted protein, homolog YXKC Bacillus subtilis (NCBI ptt file) 152, 348
CAC2069 CAC2069 Hypothetical protein (NCBI ptt file) 152, 176
CAC2094 efp Translation elongation factor P (NCBI ptt file) 152, 281
CAC2275 apt Adenine phosphoribosyltransferase; Apt (NCBI ptt file) 152, 276
CAC2295 CAC2295 Uncharacterized conserved protein, YebC family (NCBI ptt file) 152, 296
CAC2366 CAC2366 Predicted membrane protein (NCBI ptt file) 85, 152
CAC2417 CAC2417 Hypothetical protein (NCBI ptt file) 152, 162
CAC2475 CAC2475 Possible 5-Nitroimidazole antibiotics resistance protein, NimA-family (NCBI ptt file) 261, 276
CAC2546 CAC2546 Transcriptional regulator, FadR family (NCBI ptt file) 185, 276
CAC2622 comE ComE-like protein, Metallo beta-lactamase superfamily hydrolase (NCBI ptt file) 152, 233
CAC2967 CAC2967 Alpha-acetolactate decarboxylase (NCBI ptt file) 152, 206
CAC2999 CAC2999 Hypothetical protein (NCBI ptt file) 52, 276
CAC3001 CAC3001 Uncharacterized consrved protein, containing Zn finger (NCBI ptt file) 1, 276
CAC3347 CAC3347 Amino acid transporter, permease (NCBI ptt file) 207, 276
CAC3348 CAC3348 Possible homocysteine S-methyltransferase (NCBI ptt file) 157, 276
CAC3580 CAC3580 Dioxygenase related to 2-nitropropane dioxygenase (NCBI ptt file) 31, 276
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC2275
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend