Organism : Desulfovibrio vulgaris Hildenborough | Module List :
DVU3142

sigma-54 dependent transcriptional regulator

CircVis
Functional Annotations (10)
Function System
Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains cog/ cog
two-component sensor activity go/ molecular_function
two-component signal transduction system (phosphorelay) go/ biological_process
sequence-specific DNA binding transcription factor activity go/ molecular_function
ATP binding go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
transcription factor binding go/ molecular_function
nucleoside-triphosphatase activity go/ molecular_function
sensory_box tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for DVU3142
(Mouseover regulator name to see its description)

DVU3142 is regulated by 24 influences and regulates 79 modules.
Regulators for DVU3142 (24)
Regulator Module Operator
DVU0525 203 tf
DVU0653 203 tf
DVU0804
DVU0230
203 combiner
DVU1063 203 tf
DVU1083
DVU2832
203 combiner
DVU1754 203 tf
DVU2036 203 tf
DVU2036
DVU2097
203 combiner
DVU2423 203 tf
DVU2423
DVU2832
203 combiner
DVU2588
DVU0621
203 combiner
DVU2588
DVU1083
203 combiner
DVU2836
DVU0653
203 combiner
DVUA0024 203 tf
DVUA0143 203 tf
DVU0619
DVU2832
197 combiner
DVU0744
DVU0230
197 combiner
DVU1572 197 tf
DVU1572
DVU3255
197 combiner
DVU1754
DVU0619
197 combiner
DVU1754
DVU0804
197 combiner
DVU2690 197 tf
DVU2832 197 tf
DVU3305
DVU2832
197 combiner

Warning: DVU3142 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.
Click on the RegPredict links to explore the motif in RegPredict.

Motif Table (4)
Motif Id e-value Consensus Motif Logo RegPredict
377 1.10e+04 ACTTGAaaaTaTATTA
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RegPredict
378 1.30e+04 tgaaAcgAgGAGAa
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RegPredict
389 1.10e+03 CGccGtaTGCC
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RegPredict
390 4.10e+03 AcgcatCcgttaaC.TtA
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RegPredict
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for DVU3142

DVU3142 is enriched for 10 functions in 3 categories.
Enrichment Table (10)
Function System
Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains cog/ cog
two-component sensor activity go/ molecular_function
two-component signal transduction system (phosphorelay) go/ biological_process
sequence-specific DNA binding transcription factor activity go/ molecular_function
ATP binding go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
transcription factor binding go/ molecular_function
nucleoside-triphosphatase activity go/ molecular_function
sensory_box tigr/ tigrfam
Module neighborhood information for DVU3142

DVU3142 has total of 28 gene neighbors in modules 197, 203
Gene neighbors (28)
Gene Common Name Description Module membership
DVU0621 sigma-54 dependent DNA-binding response regulator 15, 197
DVU1385 hypothetical protein DVU1385 202, 203
DVU1431 hpt domain-containing protein/STAS domain-containing protein 202, 203
DVU1724 phage protein 197, 340
DVU1836 tRNA nucleotidyltransferase 202, 203
DVU2062 ATP-dependent DNA helicase UvrD 203, 289
DVU2218 GTP-binding protein 203, 264
DVU2295 methyl-accepting chemotaxis protein 202, 203
DVU2424 hypothetical protein DVU2424 203, 345
DVU2517 hypothetical protein DVU2517 203, 234
DVU2692 hypothetical protein DVU2692 182, 197
DVU2706 hypothetical protein DVU2706 197, 253
DVU2881 P4 family phage/plasmid primase 2, 197
DVU2906 umuC umuC protein 15, 203
DVU3132 glycerol-3-phosphate dehydrogenase, FAD-dependent 31, 203
DVU3142 sigma-54 dependent transcriptional regulator 197, 203
DVUA0012 nifD nitrogenase molybdenum-iron protein subunit alpha 197, 335
DVUA0031 hypothetical protein DVUA0031 115, 197
DVUA0032 hypothetical protein DVUA0032 136, 197
DVUA0034 hypothetical protein DVUA0034 197, 340
DVUA0035 hypothetical protein DVUA0035 61, 197
DVUA0038 capsular polysaccharide transport protein 197, 340
DVUA0042 putative lipoprotein 197, 328
DVUA0054 glycosyl transferase, group 1 family protein 197, 340
DVUA0093 chromate transport family protein 97, 197
DVUA0102 escT type III secretion inner membrane protein 131, 197
DVUA0128 hypothetical protein DVUA0128 131, 197
DVUA0136 zupT zinc transporter ZupT 138, 197
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for DVU3142
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend