Organism : Geobacter sulfurreducens | Module List :
GSU0366

transcriptional regulator, putative (VIMSS)

CircVis
Functional Annotations (3)
Function System
Predicted transcriptional regulators cog/ cog
calcium ion binding go/ molecular_function
sequence-specific DNA binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for GSU0366
(Mouseover regulator name to see its description)

GSU0366 is regulated by 16 influences and regulates 37 modules.
Regulators for GSU0366 (16)
Regulator Module Operator
GSU0366 280 tf
GSU0735 280 tf
GSU0811 280 tf
GSU1270 280 tf
GSU1483 280 tf
GSU1687 280 tf
GSU1727 280 tf
GSU2625 280 tf
GSU0063 45 tf
GSU0366 45 tf
GSU1483 45 tf
GSU1617 45 tf
GSU2625 45 tf
GSU3298 45 tf
GSU3387 45 tf
GSU3396 45 tf

Warning: GSU0366 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2250 2.00e+03 CC.ggca..cGGGatagaggAgaT
Loader icon
2251 1.40e+04 caatatCaacCAACAagGaggca
Loader icon
2720 6.20e+01 CCg.aTccAcGga
Loader icon
2721 1.00e+03 aTcAAtCt.gttActTGAaT
Loader icon
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for GSU0366

GSU0366 is enriched for 3 functions in 3 categories.
Enrichment Table (3)
Function System
Predicted transcriptional regulators cog/ cog
calcium ion binding go/ molecular_function
sequence-specific DNA binding go/ molecular_function
Module neighborhood information for GSU0366

GSU0366 has total of 37 gene neighbors in modules 45, 280
Gene neighbors (37)
Gene Common Name Description Module membership
GSU0141 GSU0141 conserved hypothetical protein (NCBI) 150, 280
GSU0184 b2690 HAD-superfamily hydrolase, subfamily IA, variant 1 (NCBI) 45, 234
GSU0279 GSU0279 cadherin domain/calx-beta domain protein (NCBI) 45, 253
GSU0366 GSU0366 transcriptional regulator, putative (VIMSS) 45, 280
GSU0367 GSU0367 radical SAM protein, TIGR01212 family (NCBI) 145, 280
GSU0404 GSU0404 conserved hypothetical protein (VIMSS) 251, 280
GSU0408 flgC flagellar basal-body rod protein FlgC (NCBI) 280, 312
GSU0472 GSU0472 conserved hypothetical protein (VIMSS) 251, 280
GSU0596 GSU0596 response regulator (NCBI) 45, 86
GSU0597 GSU0597 hypothetical protein (VIMSS) 45, 59
GSU0683 GSU0683 methyl-accepting chemotaxis protein, putative (VIMSS) 45, 79
GSU0696 GSU0696 glucose 1-dehydrogenase (VIMSS) 188, 280
GSU0891 GSU0891 subtilase domain protein (NCBI) 45, 218
GSU0917 GSU0917 conserved hypothetical protein (VIMSS) 45, 294
GSU0936 GSU0936 glutamine amidotransferase, class I (NCBI) 150, 280
GSU0996 GSU0996 hypothetical protein (VIMSS) 251, 280
GSU1303 GSU1303 methyl-accepting chemotaxis protein (VIMSS) 45, 234
GSU1532 nrd conserved domain protein (NCBI) 45, 165
GSU1533 recC exodeoxyribonuclease V, gamma subunit (NCBI) 45, 266
GSU1534 recB exodeoxyribonuclease V, beta subunit (NCBI) 15, 45
GSU1582 bioA adenosylmethionine--8-amino-7-oxononanoate aminotransferase (VIMSS) 120, 280
GSU1617 lexA-2 LexA repressor (NCBI) 45, 216
GSU1711 GSU1711 integrative genetic element Gsu5, resolvase (VIMSS) 45, 104
GSU2143 GSU2143 hypothetical protein (VIMSS) 280, 324
GSU2148 GSU2148 hypothetical protein (VIMSS) 188, 280
GSU2349 GSU2349 hypothetical protein (VIMSS) 21, 45
GSU2530 GSU2530 GTPase YjeQ (NCBI) 45, 141
GSU2536 GSU2536 dienelactone hydrolase family protein (VIMSS) 188, 280
GSU2828 GSU2828 GGDEF domain protein (VIMSS) 235, 280
GSU2908 GSU2908 hypothetical protein (VIMSS) 166, 280
GSU2909 GSU2909 lipoprotein, putative (VIMSS) 259, 280
GSU2934 GSU2934 cytochrome c family protein (NCBI) 45, 266
GSU2935 GSU2935 cytochrome c family protein (NCBI) 45, 133
GSU2960 modC molybdenum ABC transporter, ATP-binding protein modC (NCBI) 251, 280
GSU2988 GSU2988 conserved hypothetical protein (VIMSS) 45, 193
GSU3032 GSU3032 hypothetical protein (VIMSS) 45, 234
GSU3144 GSU3144 conserved hypothetical protein (VIMSS) 251, 280
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for GSU0366
Please add your comments for this gene by using the form below. Your comments will be publicly available.

comments powered by Disqus

Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend