Organism : Geobacter sulfurreducens | Module List :
GSU2149

transcriptional regulator, ArsR family (VIMSS)

CircVis
Functional Annotations (4)
Function System
Predicted transcriptional regulators cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for GSU2149
(Mouseover regulator name to see its description)

GSU2149 is regulated by 22 influences and regulates 18 modules.
Regulators for GSU2149 (22)
Regulator Module Operator
GSU0041 2 tf
GSU0187 2 tf
GSU0581 2 tf
GSU0732 2 tf
GSU1495 2 tf
GSU1525 2 tf
GSU1626 2 tf
GSU1727 2 tf
GSU1934 2 tf
GSU2149 2 tf
GSU2581 2 tf
GSU3041 2 tf
GSU3045 2 tf
GSU0013 271 tf
GSU0031 271 tf
GSU0041 271 tf
GSU0732 271 tf
GSU2149 271 tf
GSU3041 271 tf
GSU3045 271 tf
GSU3053 271 tf
GSU3229 271 tf

Warning: GSU2149 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2164 1.40e+01 GccGGtTT
Loader icon
2165 2.60e+02 CgtgTCaagGAGgt
Loader icon
2702 1.70e+03 tCtctaTtttTgagAGggaG
Loader icon
2703 3.60e+03 AgTGAaaaGcttGgC
Loader icon
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for GSU2149

GSU2149 is enriched for 4 functions in 3 categories.
Enrichment Table (4)
Function System
Predicted transcriptional regulators cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
Module neighborhood information for GSU2149

GSU2149 has total of 24 gene neighbors in modules 2, 271
Gene neighbors (24)
Gene Common Name Description Module membership
GSU0050 GSU0050 hypothetical protein (VIMSS) 2, 246
GSU0128 priA primosomal protein n` (NCBI) 2, 134
GSU0416 GSU0416 hypothetical protein (VIMSS) 246, 271
GSU0417 flgD flagellar hook assembly protein FlgD (NCBI) 246, 271
GSU0418 GSU0418 conserved hypothetical protein (VIMSS) 259, 271
GSU0419 flgE flagellar hook protein FlgE (NCBI) 259, 271
GSU0675 GSU0675 hypothetical protein (VIMSS) 2, 270
GSU0700 GSU0700 sensory box/response regulator (VIMSS) 2, 288
GSU0709 GSU0709 hypothetical protein (VIMSS) 2, 288
GSU0804 WrbA trp repressor binding protein WrbA (NCBI) 2, 177
GSU2149 GSU2149 transcriptional regulator, ArsR family (VIMSS) 2, 271
GSU2528 GSU2528 conserved domain protein (NCBI) 2, 177
GSU2662 GSU2662 membrane protein, putative (VIMSS) 2, 134
GSU2884 GSU2884 cytochrome c family protein (VIMSS) 2, 97
GSU2885 GSU2885 NHL repeat domain protein (VIMSS) 2, 97
GSU2912 GSU2912 cytochrome c family protein (VIMSS) 2, 166
GSU3033 GSU3033 hypothetical protein (VIMSS) 259, 271
GSU3040 yviF conserved hypothetical protein (NCBI) 185, 271
GSU3041 csrA carbon storage regulator (NCBI) 185, 271
GSU3042 flgL flagellar hook-associated protein FlgL (NCBI) 185, 271
GSU3043 flgK flagellar hook-associated protein FlgK (NCBI) 2, 271
GSU3044 GSU3044 hypothetical protein (VIMSS) 2, 271
GSU3046 flgJ flagellar protein FlgJ-like protein (NCBI) 271, 312
GSU3329 GSU3329 radical SAM domain protein (NCBI) 2, 177
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for GSU2149
Please add your comments for this gene by using the form below. Your comments will be publicly available.

comments powered by Disqus

Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend