Organism : Geobacter sulfurreducens | Module List :
GSU0031 hrcA

heat-inducible transcription repressor HrcA (NCBI)

CircVis
Functional Annotations (3)
Function System
Transcriptional regulator of heat shock gene cog/ cog
regulation of transcription, DNA-dependent go/ biological_process
hrcA tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for GSU0031
(Mouseover regulator name to see its description)

GSU0031 is regulated by 20 influences and regulates 36 modules.
Regulators for GSU0031 hrcA (20)
Regulator Module Operator
GSU0655 184 tf
GSU0776 184 tf
GSU1419 184 tf
GSU2033 184 tf
GSU2202 184 tf
GSU2670 184 tf
GSU2753 184 tf
GSU3396 184 tf
GSU0031 234 tf
GSU0300 234 tf
GSU0581 234 tf
GSU0682 234 tf
GSU1483 234 tf
GSU1617 234 tf
GSU1692 234 tf
GSU2753 234 tf
GSU2817 234 tf
GSU3045 234 tf
GSU3053 234 tf
GSU3324 234 tf

Warning: GSU0031 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2528 1.70e-05 cAAtccGgTTAtAcTtCaaCgcaa
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2529 1.70e-04 aACCCTgTgGATtacgaAaG.gTG
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2628 1.20e+04 GGcacGtGGcc.tgA.gaGCgg
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2629 6.70e+03 tTgAggggaGacCgtCcGGggGc
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for GSU0031

GSU0031 is enriched for 3 functions in 3 categories.
Enrichment Table (3)
Function System
Transcriptional regulator of heat shock gene cog/ cog
regulation of transcription, DNA-dependent go/ biological_process
hrcA tigr/ tigrfam
Module neighborhood information for GSU0031

GSU0031 has total of 44 gene neighbors in modules 184, 234
Gene neighbors (44)
Gene Common Name Description Module membership
GSU0025 GSU0025 tolB protein (NCBI) 125, 184
GSU0026 GSU0026 TonB-dependent receptor, putative (VIMSS) 57, 184
GSU0028 tolQ tolQ protein (NCBI) 184, 266
GSU0029 GSU0029 hydrolase, carbon-nitrogen family (VIMSS) 57, 184
GSU0031 hrcA heat-inducible transcription repressor HrcA (NCBI) 184, 234
GSU0032 grpE GrpE protein (NCBI) 184, 266
GSU0033 dnaK chaperone protein dnaK (NCBI) 57, 184
GSU0034 dnaJ chaperone protein dnaJ (NCBI) 57, 184
GSU0184 b2690 HAD-superfamily hydrolase, subfamily IA, variant 1 (NCBI) 45, 234
GSU0203 GSU0203 conserved hypothetical protein (VIMSS) 194, 234
GSU0409 fliE flagellar hook-basal body complex protein FliE (NCBI) 41, 234
GSU0421 fliM flagellar motor switch protein FliM (NCBI) 234, 244
GSU0422 fliN flagellar motor switch protein FliN (NCBI) 181, 234
GSU0684 cheW-2 purine-binding chemotaxis protein CheW (NCBI) 100, 234
GSU0706 GSU0706 major facilitator family transporter (VIMSS) 234, 275
GSU0707 sugE sugE protein (NCBI) 95, 234
GSU0708 GSU0708 multidrug resistance protein (VIMSS) 234, 244
GSU0724 GSU0724 conserved hypothetical protein (NCBI) 49, 234
GSU0775 GSU0775 sensor histidine kinase (VIMSS) 122, 184
GSU0776 GSU0776 sigma-54 dependent DNA-binding response regulator (VIMSS) 160, 184
GSU0777 fdnG formate dehydrogenase, major subunit, selenocysteine-containing (NCBI) 113, 184
GSU0778 fdnH formate dehydrogenase, iron-sulfur subunit (NCBI) 166, 184
GSU0779 GSU0779 formate dehydrogenase, b-type cytochrome subunit, putative (VIMSS) 166, 184
GSU0780 GSU0780 formate dehydrogenase accessory protein FdhD (VIMSS) 184, 264
GSU0897 GSU0897 conserved hypothetical protein (VIMSS) 234, 244
GSU0904 GSU0904 HD domain protein (VIMSS) 234, 244
GSU1014 GSU1014 smr domain protein (VIMSS) 234, 240
GSU1171 GSU1171 conserved hypothetical protein (VIMSS) 195, 234
GSU1288 GSU1288 hypothetical protein (VIMSS) 184, 246
GSU1303 GSU1303 methyl-accepting chemotaxis protein (VIMSS) 45, 234
GSU1341 GSU1341 ABC transporter, ATP-binding protein (VIMSS) 184, 341
GSU1399 corA-1 magnesium and cobalt transport protein CorA (NCBI) 234, 240
GSU2226 era GTP-binding protein Era (NCBI) 184, 262
GSU2227 GSU2227 radical SAM domain protein (NCBI) 184, 262
GSU2228 rnc ribonuclease III (NCBI) 184, 262
GSU2229 tmk thymidylate kinase (NCBI) 184, 190
GSU2230 holB DNA polymerase III, delta prime subunit (NCBI) 184, 336
GSU2387 GSU2387 B12-binding domain (NCBI) 165, 234
GSU2593 GSU2593 ISGsu6, transposase OrfA (VIMSS) 234, 300
GSU2626 GSU2626 conserved hypothetical protein (VIMSS) 41, 234
GSU2755 GSU2755 periplasmic substrate-binding protein/sensor histidine kinase (VIMSS) 234, 244
GSU2903 GSU2903 conserved domain protein (VIMSS) 41, 234
GSU3032 GSU3032 hypothetical protein (VIMSS) 45, 234
GSU3115 GSU3115 hypothetical protein (VIMSS) 100, 234
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for GSU0031
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend