Organism : Geobacter sulfurreducens | Module List:
Module 264 Profile

GeneModule member RegulatorRegulator MotifMotif
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 264

There are 9 regulatory influences for Module 264

Regulator Table (9)
Regulator Name Type
GSU0041 tf
GSU2735 tf
GSU2915 tf
GSU2868 tf
GSU1639 tf
GSU1992 tf
GSU1569 tf
GSU2809 tf
GSU1320 tf

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
2688 1.80e+02 AAaAAT
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2689 2.50e+03 tgtcatAaaAaaGattactT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 264 is enriched for following functions.

KEGG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini Hochberg pvalue Genes with function Method
Metabolism kegg category 2.20e-02 4.51e-02 8/28
Metabolism of Other Amino Acids kegg subcategory 6.50e-05 1.20e-03 3/28
Glycan Biosynthesis and Metabolism kegg subcategory 0.00e+00 0.00e+00 6/28
Peptidoglycan biosynthesis kegg pathway 0.00e+00 0.00e+00 6/28
Global kegg category 1.14e-02 2.93e-02 8/28
Metabolism kegg subcategory 1.14e-02 2.93e-02 8/28
Metabolic pathways kegg pathway 9.30e-03 2.53e-02 8/28

TIGRFam Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Cell envelope tigr mainrole 0.00e+00 0.00e+00 7/28
Biosynthesis and degradation of murein sacculus and peptidoglycan tigr sub1role 0.00e+00 0.00e+00 6/28

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Cellular processes and signaling cog category 4.60e-05 1.40e-04 15/28
Cell wall/membrane/envelope biogenesis cog subcategory 6.00e-06 2.40e-05 8/28
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 264

There are 28 genes in Module 264

Gene Member Table (28)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
GSU0098 GSU0098 CDS None chromosome 113608 114099 + MglB protein (VIMSS) False
GSU0388 GSU0388 CDS None chromosome 420116 420733 + conserved hypothetical protein (NCBI) False
GSU0476 GSU0476 CDS None chromosome 508371 509531 + conserved hypothetical protein (VIMSS) False
GSU0780 GSU0780 CDS None chromosome 840072 841508 + formate dehydrogenase accessory protein FdhD (VIMSS) False
GSU1117 GSU1117 CDS None chromosome 1202241 1202624 + response regulator (VIMSS) False
GSU1118 GSU1118 CDS None chromosome 1202650 1203099 + universal stress protein family (VIMSS) False
GSU1119 GSU1119 CDS None chromosome 1203159 1204421 + sensor histidine kinase/response regulator (VIMSS) False
GSU1639 GSU1639 CDS None chromosome 1797963 1798373 + Rrf2 family protein (NCBI) True
GSU1640 cydA CDS None chromosome 1798517 1799869 + cytochrome d ubiquinol oxidase, subunit I (NCBI) False
GSU1641 cydB CDS None chromosome 1799871 1800899 + cytochrome d ubiquinol oxidase, subunit II (NCBI) False
GSU1817 GSU1817 CDS None chromosome 1983011 1983580 + outer membrane lipoprotein, Slp family, putative (VIMSS) False
GSU1867 GSU1867 CDS None chromosome 2043771 2044088 - hypothetical protein (VIMSS) False
GSU1949 GSU1949 CDS None chromosome 2136680 2137354 + hypothetical protein (VIMSS) False
GSU1992 GSU1992 CDS None chromosome 2185291 2185971 - transcriptional regulator, Crp/Fnr family (VIMSS) True
GSU2044 GSU2044 CDS None chromosome 2239587 2241875 - sensory box/GGDEF family protein (VIMSS) False
GSU2730 GSU2730 CDS None chromosome 3007184 3007645 - hypothetical protein (NCBI) False
GSU3063 ftsZ CDS None chromosome 3368488 3369639 - cell division protein FtsZ (NCBI) False
GSU3065 ftsQ CDS None chromosome 3370942 3371772 - cell division protein FtsQ (NCBI) False
GSU3066 ddl CDS None chromosome 3371784 3372734 - D-alanine--D-alanine ligase (NCBI) False
GSU3067 murB CDS None chromosome 3372764 3373630 - UDP-N-acetylenolpyruvoylglucosamine reductase (NCBI) False
GSU3068 murC CDS None chromosome 3373685 3375073 - UDP-N-acetylmuramate--alanine ligase (NCBI) False
GSU3069 murG CDS None chromosome 3375076 3376170 - UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (NCBI) False
GSU3070 GSU3070 CDS None chromosome 3376167 3377231 - cell division protein, rodA/ftsW/spoVE family (VIMSS) False
GSU3071 murD CDS None chromosome 3377285 3378643 - UDP-N-acetylmuramoylalanine--D-glutamate ligase (NCBI) False
GSU3072 mraY CDS None chromosome 3378645 3379721 - phospho-N-acetylmuramoyl-pentapeptide-transferase (NCBI) False
GSU3238 GSU3238 CDS None chromosome 3551349 3551648 - Rieske 2Fe-2S family protein (VIMSS) False
GSU3240 GSU3240 CDS None chromosome 3553203 3555641 - radical SAM domain protein (NCBI) False
GSU3246 GSU3246 CDS None chromosome 3559499 3560104 - thioredoxin peroxidase (VIMSS) False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.