Organism : Geobacter sulfurreducens | Module List:
Module 290 Profile

GeneModule member RegulatorRegulator MotifMotif
Cytoscape Web
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 290

There are 6 regulatory influences for Module 290

Regulator Table (6)
Regulator Name Type
GSU2571 tf
GSU1345 tf
GSU0534 tf
GSU3206 tf
GSU2945 tf
GSU2185 tf

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
2738 4.00e+03 GgAAGGgA
Loader icon
2739 9.70e+03 tTTgtTtTcTa
Loader icon
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 290 is enriched for following functions.

KEGG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini Hochberg pvalue Genes with function Method
Metabolism kegg category 2.58e-03 1.22e-02 8/21
Amino Acid Metabolism kegg subcategory 1.30e-03 8.40e-03 4/21
Cysteine and methionine metabolism kegg pathway 1.00e-05 3.05e-04 3/21
Metabolism of Other Amino Acids kegg subcategory 2.00e-05 5.16e-04 3/21
Metabolism of Cofactors and Vitamins kegg subcategory 2.64e-03 1.23e-02 3/21
Genetic Information Processing kegg category 2.02e-02 4.25e-02 3/21
Folding Sorting and Degradation kegg subcategory 9.30e-05 1.53e-03 3/21
Sulfur relay system kegg pathway 0.00e+00 1.00e-05 3/21
Global kegg category 2.03e-02 4.25e-02 6/21
Metabolism kegg subcategory 2.03e-02 4.25e-02 6/21
Metabolic pathways kegg pathway 1.72e-02 3.80e-02 6/21

TIGRFam Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
rrf2_super tigrfam 0.00e+00 0.00e+00 3/21
Unknown function tigr mainrole 1.24e-03 2.36e-03 3/21
General tigr sub1role 2.75e-04 6.20e-04 3/21
Amino acid biosynthesis tigr mainrole 3.17e-04 7.02e-04 3/21
Serine family tigr sub1role 0.00e+00 0.00e+00 3/21

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Information storage and processing cog category 2.41e-02 4.00e-02 5/21
Metabolism cog category 6.18e-03 1.15e-02 10/21
Transcription cog subcategory 6.34e-03 1.17e-02 3/21
Amino acid transport and metabolism cog subcategory 6.00e-06 2.30e-05 7/21
Predicted transcriptional regulator cog 0.00e+00 0.00e+00 3/21
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 290

There are 21 genes in Module 290

Gene Member Table (21)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
GSU0192 GSU0192 CDS None chromosome 204369 204611 + hypothetical protein (VIMSS) False
GSU0304 pepN CDS None chromosome 334411 337044 - aminopeptidase N (NCBI) False
GSU0496 GSU0496 CDS None chromosome 528747 529955 + efflux transporter, RND family, MFP subunit (NCBI) False
GSU0534 GSU0534 CDS None chromosome 566814 567278 + RrF2 family protein (VIMSS) True
GSU0535 cysK CDS None chromosome 567299 568222 + cysteine synthase A (NCBI) False
GSU0536 GSU0536 CDS None chromosome 568286 569095 + conserved hypothetical protein TIGR00268 (VIMSS) False
GSU0537 GSU0537 CDS None chromosome 569343 571391 + sensory box/GGDEF family protein (VIMSS) False
GSU1183 mdeA CDS None chromosome 1290337 1291623 + O-acetyl-L-homoserine sulfhydrylase (NCBI) False
GSU1345 GSU1345 CDS None chromosome 1472572 1473033 + RrF2 family protein (VIMSS) True
GSU1705 panB CDS None chromosome 1868888 1869691 + 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI) False
GSU1707 GSU1707 CDS None chromosome 1870600 1872258 + group II decarboxylase (VIMSS) False
GSU1708 GSU1708 CDS None chromosome 1872319 1873581 + chlorohydrolase, Atz/Trz family (VIMSS) False
GSU1709 smpB CDS None chromosome 1873660 1874121 + SsrA-binding protein (NCBI) False
GSU2011 GSU2011 CDS None chromosome 2203783 2204958 - cysteine desulfurase (VIMSS) False
GSU2012 GSU2012 CDS None chromosome 2204955 2205812 - NifU family protein (NCBI) False
GSU2568 GSU2568 CDS None chromosome 2829875 2831179 - MiaB-like tRNA modifying enzyme (NCBI) False
GSU2569 trmU CDS None chromosome 2831179 2832240 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (NCBI) False
GSU2570 spl1 CDS None chromosome 2832260 2833444 - cysteine desulfurase (NCBI) False
GSU2571 GSU2571 CDS None chromosome 2833441 2833878 - RrF2 family protein (VIMSS) True
GSU2572 cysE CDS None chromosome 2833882 2834559 - serine acetyltransferase (NCBI) False
GSU3111 GSU3111 CDS None chromosome 3413770 3414057 - hypothetical protein (VIMSS) False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Comments for Module 290

Please add your comments for this module by using the form below. Your comments will be publicly available.

comments powered by Disqus
Social Help

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.