Organism : Pseudomonas aeruginosa | Module List :
PA1200

hypothetical protein (NCBI)

CircVis
Functional Annotations (7)
Function System
Predicted O-methyltransferase cog/ cog
O-methyltransferase activity go/ molecular_function
Histidine metabolism kegg/ kegg pathway
Tyrosine metabolism kegg/ kegg pathway
Selenocompound metabolism kegg/ kegg pathway
Polycyclic aromatic hydrocarbon degradation kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA1200
(Mouseover regulator name to see its description)

PA1200 is regulated by 32 influences and regulates 0 modules.
Regulators for PA1200 (32)
Regulator Module Operator
PA0133 68 tf
PA0253 68 tf
PA0675 68 tf
PA1067 68 tf
PA1141 68 tf
PA1145 68 tf
PA1197 68 tf
PA1374 68 tf
PA1945 68 tf
PA2534 68 tf
PA2877 68 tf
PA3002 68 tf
PA4174 68 tf
PA4270 68 tf
PA4989 68 tf
PA0376 551 tf
PA0762 551 tf
PA0763 551 tf
PA0765 551 tf
PA0784 551 tf
PA1097 551 tf
PA1520 551 tf
PA2016 551 tf
PA2047 551 tf
PA2577 551 tf
PA2586 551 tf
PA2622 551 tf
PA2737 551 tf
PA4315 551 tf
PA4493 551 tf
PA5253 551 tf
PA5483 551 tf

Warning: PA1200 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2968 6.70e-14 tacgtaT..tCAagaaa.tcg.ag
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2969 2.70e-06 tTtAtCCacgcCAAgga.Ga
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3914 2.00e+00 tTgttagaa.ggacaatTct
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3915 1.50e+03 GAAtTcagAcTgaa.AaaT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA1200

PA1200 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Predicted O-methyltransferase cog/ cog
O-methyltransferase activity go/ molecular_function
Histidine metabolism kegg/ kegg pathway
Tyrosine metabolism kegg/ kegg pathway
Selenocompound metabolism kegg/ kegg pathway
Polycyclic aromatic hydrocarbon degradation kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
Module neighborhood information for PA1200

PA1200 has total of 48 gene neighbors in modules 68, 551
Gene neighbors (48)
Gene Common Name Description Module membership
PA0133 PA0133 probable transcriptional regulator (NCBI) 61, 68
PA0309 PA0309 hypothetical protein (NCBI) 221, 551
PA0655 PA0655 hypothetical protein (NCBI) 452, 551
PA0675 PA0675 probable sigma-70 factor, ECF subfamily (NCBI) 68, 500
PA0749 PA0749 hypothetical protein (NCBI) 68, 266
PA0764 mucB negative regulator for alginate biosynthesis MucB (NCBI) 273, 551
PA0765 mucC positive regulator for alginate biosynthesis MucC (NCBI) 273, 551
PA0766 mucD serine protease MucD precursor (NCBI) 273, 551
PA0784 PA0784 probable transcriptional regulator (NCBI) 523, 551
PA1066 PA1066 short chain dehydrogenase (NCBI) 68, 346
PA1067 PA1067 probable transcriptional regulator (NCBI) 68, 542
PA1097 fleQ transcriptional regulator FleQ (NCBI) 437, 551
PA1141 PA1141 probable transcriptional regulator (NCBI) 68, 229
PA1145 PA1145 probable transcriptional regulator (NCBI) 68, 140
PA1194 PA1194 probable amino acid permease (NCBI) 68, 132
PA1195 PA1195 hypothetical protein (NCBI) 68, 313
PA1196 PA1196 probable transcriptional regulator (NCBI) 68, 162
PA1197 PA1197 NAD-dependent deacetylase (NCBI) 68, 162
PA1198 PA1198 hypothetical protein (NCBI) 68, 551
PA1199 PA1199 probable lipoprotein (NCBI) 68, 551
PA1200 PA1200 hypothetical protein (NCBI) 68, 551
PA1373 fabF2 3-oxoacyl-(acyl carrier protein) synthase (NCBI) 68, 438
PA1374 PA1374 hypothetical protein (NCBI) 68, 438
PA1376 aceK bifunctional isocitrate dehydrogenase kinase/phosphatase protein (NCBI) 513, 551
PA1578 PA1578 hypothetical protein (NCBI) 68, 483
PA1642 selD selenophosphate synthetase (NCBI) 224, 551
PA1643 PA1643 hypothetical protein (NCBI) 224, 551
PA1652 PA1652 hypothetical protein (NCBI) 68, 438
PA1772 PA1772 ribonuclease activity regulator protein RraA (NCBI) 342, 551
PA1945 PA1945 probable transcriptional regulator (NCBI) 68, 136
PA2534 PA2534 probable transcriptional regulator (NCBI) 68, 288
PA2535 PA2535 probable oxidoreductase (NCBI) 68, 359
PA2707 PA2707 hypothetical protein (NCBI) 436, 551
PA2820 PA2820 hypothetical protein (NCBI) 397, 551
PA2821 PA2821 probable glutathione S-transferase (NCBI) 397, 551
PA2877 PA2877 probable transcriptional regulator (NCBI) 68, 100
PA2910 PA2910 hypothetical protein (NCBI) 68, 92
PA3672 PA3672 probable ATP-binding component of ABC transporter (NCBI) 68, 283
PA4174 PA4174 probable transcriptional regulator (NCBI) 68, 542
PA4315 mvaT transcriptional regulator MvaT, P16 subunit (NCBI) 525, 551
PA4495 PA4495 hypothetical protein (NCBI) 525, 551
PA4793 PA4793 hypothetical protein (NCBI) 513, 551
PA4832 PA4832 probable short-chain dehydrogenase (NCBI) 68, 119
PA4844 PA4844 probable chemotaxis transducer (NCBI) 68, 74
PA4924 PA4924 hypothetical protein (NCBI) 68, 283
PA4972 PA4972 hypothetical protein (NCBI) 273, 551
PA5191 PA5191 hypothetical protein (NCBI) 159, 551
PA5227 PA5227 hypothetical protein (NCBI) 499, 551
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA1200
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend