Organism : Pseudomonas aeruginosa | Module List :
PA1322

probable TonB-dependent receptor (NCBI)

CircVis
Functional Annotations (7)
Function System
Outer membrane receptor for monomeric catechols cog/ cog
receptor activity go/ molecular_function
iron ion binding go/ molecular_function
siderophore transmembrane transporter activity go/ molecular_function
siderophore transport go/ biological_process
membrane go/ cellular_component
TonB-siderophor tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA1322
(Mouseover regulator name to see its description)

PA1322 is regulated by 33 influences and regulates 0 modules.
Regulators for PA1322 (33)
Regulator Module Operator
PA0056 394 tf
PA0191 394 tf
PA0217 394 tf
PA0218 394 tf
PA0564 394 tf
PA0828 394 tf
PA1261 394 tf
PA1347 394 tf
PA1826 394 tf
PA2050 394 tf
PA3420 394 tf
PA3596 394 tf
PA3757 394 tf
PA3771 394 tf
PA3804 394 tf
PA4806 394 tf
PA5032 394 tf
PA5293 394 tf
PA5344 394 tf
PA0032 288 tf
PA1264 288 tf
PA1328 288 tf
PA1399 288 tf
PA2054 288 tf
PA2534 288 tf
PA2696 288 tf
PA3045 288 tf
PA3381 288 tf
PA3420 288 tf
PA4238 288 tf
PA4341 288 tf
PA4902 288 tf
PA5344 288 tf

Warning: PA1322 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3404 5.40e-10 tt.cTtgTtagaa.aAtt.gaaag
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3405 2.80e-03 GCgaggcAg.ataGAaa.gtCcaa
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3612 1.40e-12 aAcaacAAgAAcaA
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3613 2.10e+04 atT.aTgAtTTTT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA1322

PA1322 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Outer membrane receptor for monomeric catechols cog/ cog
receptor activity go/ molecular_function
iron ion binding go/ molecular_function
siderophore transmembrane transporter activity go/ molecular_function
siderophore transport go/ biological_process
membrane go/ cellular_component
TonB-siderophor tigr/ tigrfam
Module neighborhood information for PA1322

PA1322 has total of 42 gene neighbors in modules 288, 394
Gene neighbors (42)
Gene Common Name Description Module membership
PA0209 PA0209 triphosphoribosyl-dephosphocoenzyme-A synthase (NCBI) 24, 394
PA0210 mdcC malonate decarboxylase subunit delta (NCBI) 24, 394
PA0213 PA0213 phosphoribosyl-dephospho-CoA transferase (NCBI) 24, 394
PA0217 PA0217 probable transcriptional regulator (NCBI) 394, 537
PA0218 PA0218 probable transcriptional regulator (NCBI) 236, 394
PA0240 PA0240 probable porin (NCBI) 394, 446
PA0709 PA0709 hypothetical protein (NCBI) 288, 292
PA1210 PA1210 hypothetical protein (NCBI) 288, 546
PA1251 PA1251 probable chemotaxis transducer (NCBI) 394, 446
PA1322 PA1322 probable TonB-dependent receptor (NCBI) 288, 394
PA1328 PA1328 probable transcriptional regulator (NCBI) 119, 288
PA1329 PA1329 hypothetical protein (NCBI) 119, 288
PA1330 PA1330 short chain dehydrogenase (NCBI) 119, 288
PA1347 PA1347 probable transcriptional regulator (NCBI) 394, 546
PA1412 PA1412 hypothetical protein (NCBI) 326, 394
PA1485 PA1485 probable amino acid permease (NCBI) 294, 394
PA1486 PA1486 hypothetical protein (NCBI) 294, 394
PA1525 alkB2 alkane-1-monooxygenase 2 (NCBI) 246, 288
PA1917 PA1917 hypothetical protein (NCBI) 394, 439
PA1977 PA1977 hypothetical protein (NCBI) 211, 394
PA2017 PA2017 hypothetical protein (NCBI) 288, 546
PA2052 cynS cyanate hydratase (NCBI) 79, 288
PA2053 cynT carbonate dehydratase (NCBI) 79, 288
PA2054 cynR transcriptional regulator CynR (NCBI) 79, 288
PA2075 PA2075 hypothetical protein (NCBI) 288, 546
PA2534 PA2534 probable transcriptional regulator (NCBI) 68, 288
PA2636 PA2636 hypothetical protein (NCBI) 201, 288
PA2715 PA2715 probable ferredoxin (NCBI) 394, 425
PA2878 PA2878 hypothetical protein (NCBI) 180, 288
PA3592 PA3592 hypothetical protein (NCBI) 394, 463
PA3681 PA3681 hypothetical protein (NCBI) 394, 432
PA4009 PA4009 hypothetical protein (NCBI) 97, 288
PA4099 PA4099 hypothetical protein (NCBI) 197, 394
PA4173 PA4173 hypothetical protein (NCBI) 288, 542
PA4177 PA4177 hypothetical protein (NCBI) 394, 401
PA4361 PA4361 probable oxidoreductase (NCBI) 187, 394
PA4437 PA4437 hypothetical protein (NCBI) 97, 288
PA4654 PA4654 probable major facilitator superfamily (MFS) transporter (NCBI) 267, 288
PA4805 PA4805 hypothetical protein (NCBI) 394, 425
PA4903 PA4903 probable major facilitator superfamily (MFS) transporter (NCBI) 394, 401
PA5293 PA5293 probable transcriptional regulator (NCBI) 394, 536
PA5391 PA5391 hypothetical protein (NCBI) 267, 394
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA1322
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend