Organism : Pseudomonas aeruginosa | Module List :
PA1430 lasR

transcriptional regulator LasR (NCBI)

CircVis
Functional Annotations (5)
Function System
DNA-binding HTH domain-containing proteins cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
sequence-specific DNA binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA1430
(Mouseover regulator name to see its description)

PA1430 is regulated by 38 influences and regulates 24 modules.
Regulators for PA1430 lasR (38)
Regulator Module Operator
PA0179 205 tf
PA0797 205 tf
PA1351 205 tf
PA1430 205 tf
PA1455 205 tf
PA1754 205 tf
PA2586 205 tf
PA2896 205 tf
PA3622 205 tf
PA4296 205 tf
PA5105 205 tf
PA5261 205 tf
PA0034 166 tf
PA0436 166 tf
PA0905 166 tf
PA0961 166 tf
PA0979 166 tf
PA1229 166 tf
PA1335 166 tf
PA1430 166 tf
PA2093 166 tf
PA2299 166 tf
PA2588 166 tf
PA2622 166 tf
PA2718 166 tf
PA3002 166 tf
PA3249 166 tf
PA3689 166 tf
PA3879 166 tf
PA4269 166 tf
PA4462 166 tf
PA4659 166 tf
PA4703 166 tf
PA4831 166 tf
PA4890 166 tf
PA5261 166 tf
PA5374 166 tf
PA5483 166 tf

Warning: PA1430 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3160 5.20e-05 aTTGaTtTaAaAggaTtTtaatTt
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3161 2.90e-09 ggcAagcTGGCACagcg.ctGCaA
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3238 6.10e-05 tgttTtaaTtgaataTacaTaTac
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3239 9.10e-01 aCtTcatcGACGcctcGAaAaGGA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA1430

PA1430 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
DNA-binding HTH domain-containing proteins cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
sequence-specific DNA binding go/ molecular_function
Module neighborhood information for PA1430

PA1430 has total of 42 gene neighbors in modules 166, 205
Gene neighbors (42)
Gene Common Name Description Module membership
PA0290 PA0290 hypothetical protein (NCBI) 163, 166
PA0553 PA0553 hypothetical protein (NCBI) 91, 205
PA0565 PA0565 hypothetical protein (NCBI) 153, 205
PA0731 PA0731 hypothetical protein (NCBI) 166, 361
PA0797 PA0797 probable transcriptional regulator (NCBI) 205, 264
PA0838 PA0838 probable glutathione peroxidase (NCBI) 2, 205
PA0959 PA0959 hypothetical protein (NCBI) 205, 469
PA0960 PA0960 hypothetical protein (NCBI) 205, 469
PA1183 dctA C4-dicarboxylate transport protein (NCBI) 166, 212
PA1287 PA1287 probable glutathione peroxidase (NCBI) 166, 209
PA1430 lasR transcriptional regulator LasR (NCBI) 166, 205
PA1471 PA1471 hypothetical protein (NCBI) 11, 166
PA1571 PA1571 hypothetical protein (NCBI) 166, 361
PA1573 PA1573 hypothetical protein (NCBI) 166, 376
PA1967 PA1967 hypothetical protein (NCBI) 166, 388
PA2025 gor glutathione reductase (NCBI) 10, 205
PA2190 PA2190 hypothetical protein (NCBI) 166, 521
PA2656 PA2656 probable two-component sensor (NCBI) 205, 478
PA2657 PA2657 probable two-component response regulator (NCBI) 205, 478
PA2658 PA2658 hypothetical protein (NCBI) 205, 478
PA2659 PA2659 hypothetical protein (NCBI) 205, 478
PA2747 PA2747 hypothetical protein (NCBI) 27, 166
PA2779 PA2779 hypothetical protein (NCBI) 128, 205
PA2827 PA2827 methionine sulfoxide reductase B (NCBI) 205, 376
PA3369 PA3369 hypothetical protein (NCBI) 27, 166
PA3370 PA3370 hypothetical protein (NCBI) 27, 166
PA3371 PA3371 hypothetical protein (NCBI) 27, 166
PA3399 PA3399 hypothetical protein (NCBI) 166, 185
PA3451 PA3451 hypothetical protein (NCBI) 27, 166
PA4444 mltB1 membrane-bound lytic transglycosylase (NCBI) 166, 338
PA4523 PA4523 hypothetical protein (NCBI) 205, 452
PA4674 PA4674 hypothetical protein (NCBI) 166, 209
PA4717 PA4717 hypothetical protein (NCBI) 205, 547
PA4738 PA4738 hypothetical protein (NCBI) 27, 166
PA4739 PA4739 hypothetical protein (NCBI) 27, 166
PA5061 PA5061 hypothetical protein (NCBI) 166, 185
PA5104 PA5104 hypothetical protein (NCBI) 205, 328
PA5105 hutC histidine utilization repressor HutC (NCBI) 205, 328
PA5106 PA5106 atrazine chlorohydrolase (NCBI) 205, 328
PA5460 PA5460 hypothetical protein (NCBI) 166, 209
PA5481 PA5481 hypothetical protein (NCBI) 27, 166
PA5482 PA5482 hypothetical protein (NCBI) 27, 166
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA1430
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend