Organism : Pseudomonas aeruginosa | Module List :
PA3649

hypothetical protein (NCBI)

CircVis
Functional Annotations (7)
Function System
Predicted membrane-associated Zn-dependent proteases 1 cog/ cog
metalloendopeptidase activity go/ molecular_function
protein binding go/ molecular_function
proteolysis go/ biological_process
zinc ion binding go/ molecular_function
integral to membrane go/ cellular_component
TIGR00054 tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA3649
(Mouseover regulator name to see its description)

PA3649 is regulated by 29 influences and regulates 0 modules.
Regulators for PA3649 (29)
Regulator Module Operator
PA0123 100 tf
PA1504 100 tf
PA1776 100 tf
PA2259 100 tf
PA2877 100 tf
PA3266 100 tf
PA3285 100 tf
PA3583 100 tf
PA3898 100 tf
PA3973 100 tf
PA4296 100 tf
PA4462 100 tf
PA4745 100 tf
PA4853 100 tf
PA5438 100 tf
PA0125 126 tf
PA0455 126 tf
PA2047 126 tf
PA2692 126 tf
PA3266 126 tf
PA3341 126 tf
PA3604 126 tf
PA3622 126 tf
PA4052 126 tf
PA4275 126 tf
PA4279 126 tf
PA4451 126 tf
PA4853 126 tf
PA5438 126 tf

Warning: PA3649 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3032 1.70e+00 ggaAAaAa
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3033 4.50e+02 CGttGaCggcC
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3082 3.50e-04 C.ggTA.AatgcccGgcTttc
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3083 8.00e+03 ATCATATA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA3649

PA3649 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Predicted membrane-associated Zn-dependent proteases 1 cog/ cog
metalloendopeptidase activity go/ molecular_function
protein binding go/ molecular_function
proteolysis go/ biological_process
zinc ion binding go/ molecular_function
integral to membrane go/ cellular_component
TIGR00054 tigr/ tigrfam
Module neighborhood information for PA3649

PA3649 has total of 38 gene neighbors in modules 100, 126
Gene neighbors (38)
Gene Common Name Description Module membership
PA0128 PA0128 hypothetical protein (NCBI) 126, 135
PA0160 PA0160 hypothetical protein (NCBI) 100, 224
PA0789 PA0789 probable amino acid permease (NCBI) 100, 490
PA1206 PA1206 hypothetical protein (NCBI) 4, 100
PA1207 kefB glutathione-regulated potassium-efflux system protein KefB (NCBI) 78, 100
PA1306 PA1306 probable HIT family protein (NCBI) 100, 499
PA1590 braB branched chain amino acid transporter (NCBI) 100, 126
PA1774 cfrX CfrX protein (NCBI) 100, 195
PA1775 cmpX conserved cytoplasmic membrane protein, CmpX protein (NCBI) 100, 195
PA2045 PA2045 hypothetical protein (NCBI) 41, 100
PA2259 ptxS transcriptional regulator PtxS (NCBI) 100, 334
PA2260 PA2260 hypothetical protein (NCBI) 100, 334
PA2261 PA2261 probable 2-ketogluconate kinase (NCBI) 100, 334
PA2262 PA2262 probable 2-ketogluconate transporter (NCBI) 100, 334
PA2263 PA2263 probable 2-hydroxyacid dehydrogenase (NCBI) 100, 334
PA2692 PA2692 probable transcriptional regulator (NCBI) 101, 126
PA2769 PA2769 hypothetical protein (NCBI) 126, 212
PA2831 PA2831 hypothetical protein (NCBI) 100, 263
PA2877 PA2877 probable transcriptional regulator (NCBI) 68, 100
PA3048 PA3048 hypothetical protein (NCBI) 126, 306
PA3109 PA3109 hypothetical protein (NCBI) 41, 126
PA3267 PA3267 hypothetical protein (NCBI) 100, 195
PA3583 glpR glycerol-3-phosphate regulon repressor (NCBI) 100, 358
PA3603 dgkA diacylglycerol kinase (NCBI) 126, 170
PA3649 PA3649 hypothetical protein (NCBI) 100, 126
PA3816 cysE O-acetylserine synthase (NCBI) 126, 409
PA3941 PA3941 hypothetical protein (NCBI) 100, 550
PA3942 tesB acyl-CoA thioesterase II (NCBI) 100, 398
PA4045 PA4045 hypothetical protein (NCBI) 126, 151
PA4046 PA4046 hypothetical protein (NCBI) 1, 126
PA4047 ribA GTP cyclohydrolase II protein (NCBI) 126, 232
PA4278 PA4278 hypothetical protein (NCBI) 126, 474
PA4279 PA4279 hypothetical protein (NCBI) 126, 474
PA4291 PA4291 hypothetical protein (NCBI) 100, 103
PA4422 PA4422 hypothetical protein (NCBI) 126, 232
PA4961 PA4961 hypothetical protein (NCBI) 40, 100
PA5485 PA5485 hypothetical protein (NCBI) 126, 229
PA5486 PA5486 hypothetical protein (NCBI) 126, 247
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA3649
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend