Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_1072 moaD

putative molybdopterin MPT converting factor, subunit 1 protein (NCBI)

CircVis
Functional Annotations (5)
Function System
Molybdopterin converting factor, small subunit cog/ cog
Mo-molybdopterin cofactor biosynthetic process go/ biological_process
sulfur compound metabolic process go/ biological_process
Sulfur relay system kegg/ kegg pathway
moaD tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_1072
(Mouseover regulator name to see its description)

RSP_1072 is regulated by 38 influences and regulates 0 modules.
Regulators for RSP_1072 moaD (38)
Regulator Module Operator
RSP_0122 240 tf
RSP_0386 240 tf
RSP_0547 240 tf
RSP_0623 240 tf
RSP_0755 240 tf
RSP_1606 240 tf
RSP_1776 240 tf
RSP_1871 240 tf
RSP_2165 240 tf
RSP_2346 240 tf
RSP_2610 240 tf
RSP_2838 240 tf
RSP_2840 240 tf
RSP_2950 240 tf
RSP_3179 240 tf
RSP_3680 240 tf
RSP_0327 154 tf
RSP_0457 154 tf
RSP_0527 154 tf
RSP_0755 154 tf
RSP_1191 154 tf
RSP_1225 154 tf
RSP_1577 154 tf
RSP_1776 154 tf
RSP_1866 154 tf
RSP_1867 154 tf
RSP_1871 154 tf
RSP_1892 154 tf
RSP_1952 154 tf
RSP_2610 154 tf
RSP_2850 154 tf
RSP_2882 154 tf
RSP_2922 154 tf
RSP_2939 154 tf
RSP_3022 154 tf
RSP_3125 154 tf
RSP_3298 154 tf
RSP_3748 154 tf

Warning: RSP_1072 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
8028 1.80e+02 atgtCTcAG
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8029 9.70e+02 TTTCCT.T
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8198 4.10e-04 tTccaggcAAa
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8199 1.10e+03 aaAAttTgCAT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_1072

RSP_1072 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Molybdopterin converting factor, small subunit cog/ cog
Mo-molybdopterin cofactor biosynthetic process go/ biological_process
sulfur compound metabolic process go/ biological_process
Sulfur relay system kegg/ kegg pathway
moaD tigr/ tigrfam
Module neighborhood information for RSP_1072

RSP_1072 has total of 32 gene neighbors in modules 154, 240
Gene neighbors (32)
Gene Common Name Description Module membership
RSP_0332 RSP_0332 possible cellulose synthase (NCBI) 240, 259
RSP_0387 RSP_0387 hypothetical protein (NCBI) 154, 294
RSP_0726 RSP_0726 arginosuccinate lyase 1 (NCBI) 51, 240
RSP_0755 nusB putative N utilization substance protein B (NCBI) 234, 240
RSP_0763 aatA aspartate aminotransferase A (NCBI) 162, 240
RSP_0782 RSP_0782 Ppx/GppA phosphatase (NCBI) 215, 240
RSP_0783 RSP_0783 hypothetical protein (NCBI) 116, 240
RSP_0784 RSP_0784 putative acetyltransferase, GNAT family (NCBI) 215, 240
RSP_0841 RSP_0841 hypothetical protein (NCBI) 218, 240
RSP_0931 ialA NUDIX hydrolase (NCBI) 23, 240
RSP_1071 moaE Molybdopterin converting factor subunit 2 (NCBI) 154, 208
RSP_1072 moaD putative molybdopterin MPT converting factor, subunit 1 protein (NCBI) 154, 240
RSP_1098 RSP_1098 hypothetical protein (NCBI) 240, 262
RSP_1483 RSP_1483 hypothetical protein (NCBI) 240, 293
RSP_1775 RSP_1775 hypothetical protein (NCBI) 7, 240
RSP_1776 greA Transcription elongation factor greA (NCBI) 240, 372
RSP_1839 RSP_1839 putative acetyltransferase, GNAT family (NCBI) 240, 337
RSP_1850 ptsI phosphoenolpyruvate-protein phosphotransferase, PtsI(Ntr) (NCBI) 213, 240
RSP_1893 RSP_1893 Inositol monophosphatase family protein (NCBI) 214, 240
RSP_2272 ampD N-acetylmuramoyl-L-alanine amidase, family 2 (NCBI) 154, 294
RSP_2295 RSP_2295 hypothetical protein (NCBI) 23, 154
RSP_2616 RSP_2616 hypothetical protein (NCBI) 223, 240
RSP_2629 gid Glucose-inhibited division protein A (NCBI) 65, 240
RSP_2698 RSP_2698 putative quinoprotein (NCBI) 166, 240
RSP_2716 lpxB putative lipid-A-disaccharide synthase (NCBI) 7, 240
RSP_2784 cycG Diheme class I cytochrome c (NCBI) 240, 274
RSP_2785 CycF Cytochrome c-554 (NCBI) 240, 274
RSP_2812 RSP_2812 hypothetical protein (NCBI) 87, 240
RSP_2836 nifR3 tRNA-dihydrouridine synthase, nifR3 (NCBI) 240, 318
RSP_2960 RSP_2960 pantetheine-phosphate adenylyltransferase (NCBI) 223, 240
RSP_3376 aroQ 3 dehydroquinase dehydratase, class II (NCBI) 240, 381
RSP_3823 proB1 glutamate 5-kinase (NCBI) 30, 240
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend