Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_1357

cytoplasmic sensor hybrid histidine kinase (NCBI)

CircVis
Functional Annotations (8)
Function System
Signal transduction histidine kinase cog/ cog
two-component sensor activity go/ molecular_function
two-component response regulator activity go/ molecular_function
two-component signal transduction system (phosphorelay) go/ biological_process
ATP binding go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
membrane go/ cellular_component
peptidyl-histidine phosphorylation go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_1357
(Mouseover regulator name to see its description)

RSP_1357 is regulated by 31 influences and regulates 0 modules.
Regulators for RSP_1357 (31)
Regulator Module Operator
RSP_0607 137 tf
RSP_0641 137 tf
RSP_1014 137 tf
RSP_1286 137 tf
RSP_1607 137 tf
RSP_1776 137 tf
RSP_1790 137 tf
RSP_1871 137 tf
RSP_2867 137 tf
RSP_2889 137 tf
RSP_3024 137 tf
RSP_3064 137 tf
RSP_3124 137 tf
RSP_3322 137 tf
RSP_3385 137 tf
RSP_3464 137 tf
RSP_3684 137 tf
RSP_1014 74 tf
RSP_1518 74 tf
RSP_1590 74 tf
RSP_1660 74 tf
RSP_2351 74 tf
RSP_2494 74 tf
RSP_2533 74 tf
RSP_2780 74 tf
RSP_2867 74 tf
RSP_2963 74 tf
RSP_3064 74 tf
RSP_3322 74 tf
RSP_3464 74 tf
RSP_3684 74 tf

Warning: RSP_1357 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7868 5.20e+01 c..cc..AggAacAG
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7869 1.60e+02 tCctcGACaTg
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7994 9.60e+00 GaTcc.TtTC
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7995 2.50e-01 CCGGaatTCcT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_1357

RSP_1357 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
Signal transduction histidine kinase cog/ cog
two-component sensor activity go/ molecular_function
two-component response regulator activity go/ molecular_function
two-component signal transduction system (phosphorelay) go/ biological_process
ATP binding go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
membrane go/ cellular_component
peptidyl-histidine phosphorylation go/ biological_process
Module neighborhood information for RSP_1357

RSP_1357 has total of 50 gene neighbors in modules 74, 137
Gene neighbors (50)
Gene Common Name Description Module membership
RSP_0151 RSP_0151 hypothetical protein (NCBI) 38, 74
RSP_0152 RSP_0152 P-loop ATPase (NCBI) 74, 280
RSP_0211 RSP_0211 hypothetical protein (NCBI) 74, 191
RSP_0212 RSP_0212 HNH nuclease (NCBI) 74, 193
RSP_0330 RSP_0330 hypothetical protein (NCBI) 74, 116
RSP_0413 RSP_0413 two component diguanylate cyclase (NCBI) 137, 236
RSP_0515 RSP_0515 hypothetical protein (NCBI) 137, 217
RSP_0516 RSP_0516 hypothetical protein (NCBI) 137, 217
RSP_0517 RSP_0517 hypothetical protein (NCBI) 137, 217
RSP_0518 RSP_0518 putative glycosyltransferase protein (NCBI) 137, 217
RSP_0523 RSP_0523 hypothetical protein (NCBI) 137, 217
RSP_0524 RSP_0524 hypothetical protein (NCBI) 74, 168
RSP_0919 RSP_0919 Short-chain dehydrogenase/reductase SDR (NCBI) 137, 150
RSP_0946 RSP_0946 putative DNA topoisomerase I protein (NCBI) 74, 150
RSP_1113 RSP_1113 Hemolysin-type calcium-binding region; RTX toxin (NCBI) 74, 115
RSP_1117 RSP_1117 hypothetical protein (NCBI) 137, 246
RSP_1120 RSP_1120 protein secretion protein, HlyD family, membrane fusion protein (NCBI) 137, 246
RSP_1195 comF Competence protein F (NCBI) 74, 150
RSP_1357 RSP_1357 cytoplasmic sensor hybrid histidine kinase (NCBI) 74, 137
RSP_1501 RSP_1501 Antisigma-factor antagonist (STAS) domain protein (NCBI) 74, 292
RSP_1530 RSP_1530 hypothetical protein (NCBI) 137, 196
RSP_1821 RSP_1821 Molybdopterin-containing oxidoreductase, probable formate dehydrogenase (NCBI) 38, 137
RSP_2067 RSP_2067 putative prophage LambdaSo, major capsid protein, HK97 family (NCBI) 137, 191
RSP_2069 RSP_2069 putative phage portal protein, HK97 family (NCBI) 137, 191
RSP_2177 RSP_2177 hypothetical protein (NCBI) 137, 191
RSP_2292 RSP_2292 None 74, 137
RSP_2351 RSP_2351 hypothetical protein (NCBI) 74, 362
RSP_2352 RSP_2352 putative terminase large subunit / phage terminase (NCBI) 74, 83
RSP_2353 RSP_2353 hypothetical protein (NCBI) 74, 83
RSP_2497 RSP_2497 ATPase (NCBI) 74, 238
RSP_2498 RSP_2498 None 74, 300
RSP_2679 RSP_2679 ATP-dependent DNA ligase (NCBI) 74, 191
RSP_2755 RSP_2755 hypothetical protein (NCBI) 74, 77
RSP_3014 RSP_3014 putative aldolase protein (NCBI) 137, 304
RSP_3016 RSP_3016 Possible phenol degradation enzyme (NCBI) 137, 300
RSP_3019 RSP_3019 putative hydrolase (NCBI) 137, 300
RSP_3021 RSP_3021 putative catechol 2,3-dioxygenase (NCBI) 137, 300
RSP_3063 RSP_3063 AMP-dependent synthetase and ligase (NCBI) 74, 137
RSP_3100 RSP_3100 hypothetical protein (NCBI) 137, 236
RSP_3206 RSP_3206 Putative xanthine dehydrogenase (NCBI) 74, 236
RSP_3353 RSP_3353 Phage related tail protein (NCBI) 74, 150
RSP_3414 RSP_3414 ABC Fe+3-siderophore transporter, inner membrane subunit (NCBI) 74, 196
RSP_3415 RSP_3415 ABC Fe+3-siderophore transporter, inner membrane subunit (NCBI) 74, 138
RSP_3416 RSP_3416 ABC Fe+3-siderophore transporter, periplasmic binding protein (NCBI) 74, 331
RSP_3427 RSP_3427 hypothetical protein (NCBI) 137, 238
RSP_3623 RSP_3623 putative integrase (NCBI) 137, 191
RSP_3682 RSP_3682 hypothetical protein (NCBI) 74, 304
RSP_3763 RSP_3763 hypothetical protein (NCBI) 137, 138
RSP_3791 RSP_3791 hypothetical protein (NCBI) 137, 138
RSP_6100 RSP_6100 None 74, 364
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_1357
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend