Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_1998

Molybdenum cofactor biosynthesis protein A (NCBI)

CircVis
Functional Annotations (2)
Function System
Molybdopterin biosynthesis enzyme cog/ cog
Mo-molybdopterin cofactor biosynthetic process go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_1998
(Mouseover regulator name to see its description)

RSP_1998 is regulated by 18 influences and regulates 0 modules.
Regulators for RSP_1998 (18)
Regulator Module Operator
RSP_1231 30 tf
RSP_2346 30 tf
RSP_0090 114 tf
RSP_0327 114 tf
RSP_0794 114 tf
RSP_1092 114 tf
RSP_1225 114 tf
RSP_1435 114 tf
RSP_1590 114 tf
RSP_2130 114 tf
RSP_2171 114 tf
RSP_2681 114 tf
RSP_2838 114 tf
RSP_2840 114 tf
RSP_2939 114 tf
RSP_2950 114 tf
RSP_3095 114 tf
RSP_3664 114 tf

Warning: RSP_1998 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7780 7.00e-02 ct.TtCtCctcaTc.c
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7781 1.10e+03 CccgcaAAgggcAAgAgcaTtc
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7948 5.60e+00 tTgAcagacaCAtTT
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7949 6.60e+01 CgGAaAAgTgttT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_1998

RSP_1998 is enriched for 2 functions in 3 categories.
Enrichment Table (2)
Function System
Molybdopterin biosynthesis enzyme cog/ cog
Mo-molybdopterin cofactor biosynthetic process go/ biological_process
Module neighborhood information for RSP_1998

RSP_1998 has total of 47 gene neighbors in modules 30, 114
Gene neighbors (47)
Gene Common Name Description Module membership
RSP_0341 RSP_0341 Cytosine deaminase (NCBI) 57, 114
RSP_0342 RSP_0342 putative ABC sugar transporter, inner membrane subunit (NCBI) 57, 114
RSP_0353 RSP_0353 None 30, 297
RSP_0371 RSP_0371 ABC basic amino acid transporter, ATPase subunit (NCBI) 67, 114
RSP_0372 RSP_0372 ABC basic amino acid transporter, solute-binding protein (NCBI) 67, 114
RSP_0373 RSP_0373 ABC basic amino acid transporter, inner membrane subunit (NCBI) 65, 114
RSP_0374 RSP_0374 ABC basic amino acid transporter, inner membrane subunit (NCBI) 65, 114
RSP_0452 recA Recombinase A, DNA recombination protein (NCBI) 30, 93
RSP_0657 RSP_0657 hypothetical protein (NCBI) 30, 72
RSP_0658 RSP_0658 Probable ATP-dependent helicase (NCBI) 30, 72
RSP_0659 RSP_0659 Putative metallo-phosphoesterase (NCBI) 30, 72
RSP_0804 DppD ABC dipeptide transporter, ATPase subunit DppD (NCBI) 67, 114
RSP_0806 DppB ABC dipeptide transporter, inner membrane subunit DppB (NCBI) 114, 204
RSP_0807 DdpA ABC dipeptide transporter, substrate-binding subunit DdpA (NCBI) 114, 204
RSP_0863 leuC 3-isopropylmalate dehydratase large subunit (NCBI) 30, 203
RSP_1360 RSP_1360 hypothetical protein (NCBI) 30, 93
RSP_1410 RSP_1410 hypothetical protein (NCBI) 2, 30
RSP_1489 RSP_1489 Protein containing thioredoxin domain (NCBI) 30, 71
RSP_1538 RSP_1538 probable glucose-1-phosphate cytidylyltransferase (NCBI) 114, 308
RSP_1539 RSP_1539 Glycosyl transferase, family 2 (NCBI) 114, 308
RSP_1568 RSP_1568 Glutamine synthetase (NCBI) 114, 374
RSP_1997 lexA LexA repressor (NCBI) 30, 93
RSP_1998 RSP_1998 Molybdenum cofactor biosynthesis protein A (NCBI) 30, 114
RSP_2000 RSP_2000 molybdenum cofactor biosynthesis protein C (NCBI) 30, 207
RSP_2154 RSP_2154 hypothetical protein (NCBI) 17, 114
RSP_2157 RSP_2157 ABC transporter, inner membrane subunit (NCBI) 105, 114
RSP_2356 RSP_2356 hypothetical protein (NCBI) 93, 114
RSP_2457 RSP_2457 hypothetical protein (NCBI) 30, 267
RSP_2460 alr alanine racemase (NCBI) 30, 369
RSP_2561 exoP putative succinoglycan biosynthesis transport protein ExoP (NCBI) 114, 200
RSP_2562 exoM succinoglycan biosynthesis protein exoM (NCBI) 114, 200
RSP_2563 exoA Glycosyl transferase, family 2 (NCBI) 114, 200
RSP_2564 exoL glycosyltransferase, Succinoglycan biosynthesis protein exoL (NCBI) 114, 200
RSP_2624 RSP_2624 hypothetical protein (NCBI) 30, 93
RSP_2715 RSP_2715 hypothetical protein (NCBI) 7, 114
RSP_2722 cysS Cysteinyl-tRNA synthetase, class Ia (NCBI) 30, 214
RSP_2844 RSP_2844 Putative GTP-binding protein (NCBI) 59, 114
RSP_2851 RSP_2851 multidrug (bicyclomycin) efflux pump, Major facilitator superfamily (MFS) (NCBI) 12, 30
RSP_3248 RSP_3248 ABC peptide transporter, periplasmic binding protein (NCBI) 57, 114
RSP_3572 galM putative aldose 1-epimerase protein (NCBI) 23, 30
RSP_3802 RSP_3802 hypothetical protein (NCBI) 114, 204
RSP_3803 RSP_3803 hypothetical protein (NCBI) 114, 204
RSP_3804 RSP_3804 hypothetical protein (NCBI) 114, 204
RSP_3808 RSP_3808 hypothetical protein (NCBI) 67, 114
RSP_3822 RSP_3822 GTP1/OBG family protein (NCBI) 30, 279
RSP_3823 proB1 glutamate 5-kinase (NCBI) 30, 240
RSP_3824 proA Gamma-glutamyl phosphate reductase GPR (NCBI) 30, 101
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

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Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

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Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

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Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

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CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
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Comments for RSP_1998
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend