Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_2129

Sensor Histidine kinase (NCBI)

CircVis
Functional Annotations (7)
Function System
Signal transduction histidine kinase regulating C4-dicarboxylate transport system cog/ cog
two-component sensor activity go/ molecular_function
ATP binding go/ molecular_function
signal transduction go/ biological_process
membrane go/ cellular_component
peptidyl-histidine phosphorylation go/ biological_process
Two-component system kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_2129
(Mouseover regulator name to see its description)

RSP_2129 is regulated by 25 influences and regulates 0 modules.
Regulators for RSP_2129 (25)
Regulator Module Operator
RSP_0316 237 tf
RSP_0386 237 tf
RSP_0415 237 tf
RSP_0907 237 tf
RSP_1577 237 tf
RSP_1660 237 tf
RSP_2130 237 tf
RSP_2236 237 tf
RSP_3094 237 tf
RSP_3238 237 tf
RSP_3317 237 tf
RSP_3341 237 tf
RSP_3464 237 tf
RSP_3621 237 tf
RSP_3665 237 tf
RSP_3694 237 tf
RSP_0386 256 tf
RSP_0395 256 tf
RSP_1055 256 tf
RSP_1191 256 tf
RSP_2171 256 tf
RSP_2922 256 tf
RSP_3124 256 tf
RSP_3298 256 tf
RSP_3606 256 tf

Warning: RSP_2129 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
8192 1.60e+01 gtgAAAcA
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8193 1.30e+03 ATaTTcT
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8230 3.80e+01 CtGgAAgcgcC
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8231 6.70e+03 GatttaaCTGAAG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_2129

RSP_2129 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Signal transduction histidine kinase regulating C4-dicarboxylate transport system cog/ cog
two-component sensor activity go/ molecular_function
ATP binding go/ molecular_function
signal transduction go/ biological_process
membrane go/ cellular_component
peptidyl-histidine phosphorylation go/ biological_process
Two-component system kegg/ kegg pathway
Module neighborhood information for RSP_2129

RSP_2129 has total of 25 gene neighbors in modules 237, 256
Gene neighbors (25)
Gene Common Name Description Module membership
RSP_0411 RSP_0411 hypothetical protein (NCBI) 197, 256
RSP_0569 RSP_0569 hypothetical protein (NCBI) 237, 366
RSP_0570 RSP_0570 hypothetical protein (NCBI) 237, 366
RSP_0665 ftsH FtsH (NCBI) 237, 365
RSP_1204 RSP_1204 ABC multidrug efflux pump, fused ATPase and inner membrane subunits (NCBI) 216, 237
RSP_1743 RSP_1743 ATPase, AAA family (NCBI) 216, 237
RSP_1845 xseA Exonuclease VII, large subunit (NCBI) 106, 256
RSP_1930 pyrC Dihydroorotase, homodimeric type (NCBI) 256, 375
RSP_1963 RSP_1963 hypothetical protein (NCBI) 256, 343
RSP_1976 cobT aerobic cobalt chelatase, CobT subunit (NCBI) 20, 256
RSP_1982 RSP_1982 Aminopeptidase N (NCBI) 4, 256
RSP_2092 RSP_2092 Putative uvrD/DNA Helicase II (NCBI) 117, 237
RSP_2103 murD UDP-N-acetylmuramoylalanine--D-glutamate ligase (NCBI) 256, 384
RSP_2129 RSP_2129 Sensor Histidine kinase (NCBI) 237, 256
RSP_2308 RSP_2308 hypothetical protein (NCBI) 36, 237
RSP_2427 cobV putative Cobalamin (5'-phosphate) synthase (NCBI) 33, 256
RSP_2505 creA possible CreA protein (NCBI) 10, 237
RSP_2540 tatA twin-arginine translocation system protein, TatA (NCBI) 48, 237
RSP_2641 RSP_2641 hypothetical protein (NCBI) 97, 237
RSP_2843 hfq Host factor I protein (NCBI) 56, 256
RSP_2952 RSP_2952 BolA-like protein (NCBI) 237, 261
RSP_2953 RSP_2953 glutaredoxin-related protein (NCBI) 23, 237
RSP_3210 qxtB Quinol oxidase subunit II QxtB (NCBI) 172, 237
RSP_3212 qxtA Quinol oxidase subunit I QxtA (NCBI) 172, 237
RSP_3562 sqr sulfide-quinone reductase (NCBI) 64, 237
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_2129
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend