Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_2192 mcmA

Methylmalonyl-CoA mutase (NCBI)

CircVis
Functional Annotations (12)
Function System
Methylmalonyl-CoA mutase, N-terminal domain/subunit cog/ cog
methylmalonyl-CoA mutase activity go/ molecular_function
metabolic process go/ biological_process
cobalamin binding go/ molecular_function
metal ion binding go/ molecular_function
Valine leucine and isoleucine degradation kegg/ kegg pathway
Glyoxylate and dicarboxylate metabolism kegg/ kegg pathway
Propanoate metabolism kegg/ kegg pathway
Carbon fixation pathways in prokaryotes kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
acid_CoA_mut_C tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_2192
(Mouseover regulator name to see its description)

RSP_2192 is regulated by 21 influences and regulates 0 modules.
Regulators for RSP_2192 mcmA (21)
Regulator Module Operator
RSP_0722 384 tf
RSP_0728 384 tf
RSP_2171 384 tf
RSP_2236 384 tf
RSP_2533 384 tf
RSP_2840 384 tf
RSP_2922 384 tf
RSP_3665 384 tf
RSP_0623 65 tf
RSP_0958 65 tf
RSP_1225 65 tf
RSP_1435 65 tf
RSP_1518 65 tf
RSP_1712 65 tf
RSP_1890 65 tf
RSP_2410 65 tf
RSP_2533 65 tf
RSP_2681 65 tf
RSP_2801 65 tf
RSP_2838 65 tf
RSP_2950 65 tf

Warning: RSP_2192 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7850 1.70e-02 att.ccTtGAA
Loader icon
7851 1.40e+02 AaCCCcTtTtCaGctTctTCgAgA
Loader icon
8458 2.40e+01 Tttctccga.GgctA
Loader icon
8459 8.70e+03 tCg.TtcAAaggcctGctcttGc
Loader icon
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_2192

RSP_2192 is enriched for 12 functions in 3 categories.
Enrichment Table (12)
Function System
Methylmalonyl-CoA mutase, N-terminal domain/subunit cog/ cog
methylmalonyl-CoA mutase activity go/ molecular_function
metabolic process go/ biological_process
cobalamin binding go/ molecular_function
metal ion binding go/ molecular_function
Valine leucine and isoleucine degradation kegg/ kegg pathway
Glyoxylate and dicarboxylate metabolism kegg/ kegg pathway
Propanoate metabolism kegg/ kegg pathway
Carbon fixation pathways in prokaryotes kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
acid_CoA_mut_C tigr/ tigrfam
Module neighborhood information for RSP_2192

RSP_2192 has total of 46 gene neighbors in modules 65, 384
Gene neighbors (46)
Gene Common Name Description Module membership
RSP_0368 RSP_0368 CTP synthase (NCBI) 65, 384
RSP_0373 RSP_0373 ABC basic amino acid transporter, inner membrane subunit (NCBI) 65, 114
RSP_0374 RSP_0374 ABC basic amino acid transporter, inner membrane subunit (NCBI) 65, 114
RSP_0375 RSP_0375 probable glutamine synthetase (NCBI) 65, 67
RSP_0407 RSP_0407 putative lactate dehydrogenase (NCBI) 175, 384
RSP_0623 RSP_0623 hypothetical protein (NCBI) 65, 263
RSP_0624 RSP_0624 hypothetical protein (NCBI) 65, 263
RSP_0625 RSP_0625 Putative Maf-like protein (NCBI) 65, 263
RSP_0713 RSP_0713 hypothetical protein (NCBI) 96, 384
RSP_0722 RSP_0722 transcriptional regulator, AsnC family (NCBI) 254, 384
RSP_0874 RSP_0874 Formylmethionine deformylase (NCBI) 47, 65
RSP_0875 fmt methionyl-tRNA formyl transferase (NCBI) 47, 65
RSP_0879 RSP_0879 hypothetical protein (NCBI) 7, 65
RSP_0894 RSP_0894 hypothetical protein (NCBI) 175, 384
RSP_1031 RSP_1031 Endonuclease III (NCBI) 110, 384
RSP_1061 RSP_1061 hypothetical protein (NCBI) 65, 208
RSP_1099 LspA signal peptidase II (NCBI) 113, 384
RSP_1161 RSP_1161 3'-Phosphadenosine 5'-phosphosulfate 3'-phosphatase (NCBI) 109, 384
RSP_1340 RSP_1340 Enoyl-CoA hydratase/isomerase (NCBI) 67, 384
RSP_1367 RSP_1367 Nicotinate phosphoribosyltransferase (NCBI) 207, 384
RSP_1368 pncA probable pyrazinamidase/nicotinamidase (NCBI) 136, 384
RSP_1379 RSP_1379 Cytosol aminopeptidase (NCBI) 175, 384
RSP_1676 era GTP-binding protein, Era-like (NCBI) 65, 224
RSP_1754 RSP_1754 hypothetical protein (NCBI) 72, 384
RSP_1983 sqdA Phospholipid/glycerol acyltransferase (NCBI) 293, 384
RSP_2042 RSP_2042 hypothetical protein (NCBI) 65, 358
RSP_2044 RSP_2044 ATPase (NCBI) 65, 358
RSP_2103 murD UDP-N-acetylmuramoylalanine--D-glutamate ligase (NCBI) 256, 384
RSP_2190 RSP_2190 hypothetical protein (NCBI) 65, 130
RSP_2192 mcmA Methylmalonyl-CoA mutase (NCBI) 65, 384
RSP_2423 RSP_2423 putative Heat shock protein 15 (HSP15) (NCBI) 65, 208
RSP_2628 gltX/glnS glutamyl-/ glutaminyl-tRNA synthetase (NCBI) 65, 267
RSP_2629 gid Glucose-inhibited division protein A (NCBI) 65, 240
RSP_2883 pgm Probable phosphoglucomutase/phosphomannomutase (NCBI) 175, 384
RSP_2908 RSP_2908 hypothetical protein (NCBI) 110, 384
RSP_2909 RSP_2909 Hydrolase arginase family (NCBI) 110, 384
RSP_2924 RSP_2924 ABC branched-chain amino acid transporter family, ATPase subunit (NCBI) 65, 208
RSP_2925 RSP_2925 ABC branched-chain amino acid transporter family, ATPase subunit (NCBI) 65, 208
RSP_2926 RSP_2926 ABC branched-chain amino acid transporter family, inner membrane subunit (NCBI) 65, 208
RSP_2927 RSP_2927 ABC branched-chain amino acid transporter family, inner membrane subunit (NCBI) 65, 263
RSP_2928 RSP_2928 hypothetical protein (NCBI) 65, 208
RSP_3246 RSP_3246 putative D-alanyl-D-alanine carboxypeptidase (NCBI) 175, 384
RSP_3507 RSP_3507 hypothetical protein (NCBI) 381, 384
RSP_3645 RSP_3645 hypothetical protein (NCBI) 65, 130
RSP_3665 RSP_3665 transcriptional regulator, LuxR family (NCBI) 214, 384
RSP_3728 RSP_3728 putative acyl carrier protein phosphodiesterase (NCBI) 354, 384
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_2192
Please add your comments for this gene by using the form below. Your comments will be publicly available.

comments powered by Disqus

Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend