Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_4049 pdhAb

Pyruvate dehydrogenase E1 component, beta subunit (NCBI)

CircVis
Functional Annotations (11)
Function System
Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit cog/ cog
pyruvate dehydrogenase (acetyl-transferring) activity go/ molecular_function
metabolic process go/ biological_process
Glycolysis / Gluconeogenesis kegg/ kegg pathway
Citrate cycle (TCA cycle) kegg/ kegg pathway
Valine leucine and isoleucine biosynthesis kegg/ kegg pathway
Pyruvate metabolism kegg/ kegg pathway
Butanoate metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_4049
(Mouseover regulator name to see its description)

RSP_4049 is regulated by 23 influences and regulates 0 modules.
Regulators for RSP_4049 pdhAb (23)
Regulator Module Operator
RSP_0014 291 tf
RSP_0316 291 tf
RSP_0327 291 tf
RSP_0402 291 tf
RSP_1139 291 tf
RSP_1191 291 tf
RSP_1231 291 tf
RSP_2130 291 tf
RSP_2591 291 tf
RSP_2681 291 tf
RSP_2853 291 tf
RSP_3202 291 tf
RSP_0327 25 tf
RSP_0698 25 tf
RSP_0728 25 tf
RSP_0755 25 tf
RSP_0927 25 tf
RSP_1231 25 tf
RSP_2610 25 tf
RSP_2867 25 tf
RSP_3202 25 tf
RSP_3203 25 tf
RSP_3464 25 tf

Warning: RSP_4049 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7770 7.30e+02 aAcGGtcAAgaacCa
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7771 4.50e+01 AcTtaaGgcGCtTcGCagaagcAt
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8296 9.20e-04 GCtTtGacgGcaccga.tTgTCgC
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8297 2.30e+02 aaGCCaCCCcaTGCGgcgtaa
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_4049

RSP_4049 is enriched for 11 functions in 3 categories.
Module neighborhood information for RSP_4049

RSP_4049 has total of 50 gene neighbors in modules 25, 291
Gene neighbors (50)
Gene Common Name Description Module membership
RSP_0097 smoM TRAP-T family sorbitol/mannitol transporter, periplasmic binding protein, SmoM (NCBI) 291, 369
RSP_0099 RSP_0099 TRAP-T family sorbitol/mannitol transporter, DctQ (4TMs) subunit (NCBI) 291, 363
RSP_0147 glnA Glutamine synthetase class-I (NCBI) 25, 93
RSP_0169 RSP_0169 Na+/solute symporter (NCBI) 183, 291
RSP_0170 RSP_0170 hypothetical protein (NCBI) 174, 291
RSP_0171 RSP_0171 Response regulator receiver protein (NCBI) 178, 291
RSP_0250 RSP_0250 hypothetical protein (NCBI) 25, 278
RSP_0550 RSP_0550 putative D-alanyl-D-alanine carboxypeptidase (NCBI) 25, 52
RSP_0576 RSP_0576 Na+/solute symporter (NCBI) 86, 291
RSP_0577 RSP_0577 hypothetical protein (NCBI) 86, 291
RSP_0745 RSP_0745 acetyl-CoA acetyltransferase (NCBI) 25, 195
RSP_0747 RSP_0747 Short-chain dehydrogenase/reductase (NCBI) 25, 195
RSP_0915 RSP_0915 hypothetical protein (NCBI) 25, 278
RSP_0943 yhxA probable adenosylmethionine-8-amino-7-oxononanoate aminotransferase (NCBI) 25, 175
RSP_0968 mdh malate dehydrogenase (RefSeq) 25, 166
RSP_1002 pyrB Aspartate/ornithine carbamoyltransferase (NCBI) 25, 224
RSP_1131 dapD 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase (NCBI) 25, 192
RSP_1342 dnaA chromosomal replication initiator protein, DnaA (NCBI) 25, 381
RSP_1343 RSP_1343 DNA polymerase III, beta chain (NCBI) 25, 372
RSP_1345 RSP_1345 hypothetical protein (NCBI) 25, 262
RSP_1593 RSP_1593 NAD dependent malic enzyme (NCBI) 15, 25
RSP_1595 RSP_1595 Thymidine phosphorylase (NCBI) 15, 25
RSP_1598 RSP_1598 putative uracil phosphoribosyltransferase (NCBI) 15, 25
RSP_1613 RSP_1613 TRAP-T family transporter, DctP subunit (NCBI) 25, 291
RSP_1614 RSP_1614 TRAP-T family transporter with fused DctQ/DctM subunits (NCBI) 25, 112
RSP_1615 RSP_1615 hypothetical protein (NCBI) 25, 112
RSP_1913 RSP_1913 3-oxoadipate CoA-transferase, beta subunit (NCBI) 25, 363
RSP_1914 RSP_1914 3-ketoacid CoA transferase alpha subunit (NCBI) 25, 363
RSP_1986 gatB Glutamyl-tRNA amidotransferase subunit B (NCBI) 25, 309
RSP_2131 rne Ribonuclease E (NCBI) 25, 223
RSP_2397 RSP_2397 ABC putrescine transporter, periplasmic substrate-binding subunit (NCBI) 70, 291
RSP_2398 RSP_2398 ABC putrescine transporter, inner membrane subunit (NCBI) 174, 291
RSP_2399 RSP_2399 ABC putrescine transporter, inner membrane subunit (NCBI) 127, 291
RSP_2465 RSP_2465 hypothetical protein (NCBI) 25, 203
RSP_2541 tatC twin-arginine translocation system protein, TatC (NCBI) 67, 291
RSP_2622 ligA DNA ligase (NCBI) 25, 98
RSP_2781 RSP_2781 peroxiredoxin/glutaredoxin family protein (NCBI) 25, 184
RSP_3553 dnaE DNA polymerase III alpha chain (NCBI) 291, 381
RSP_3554 xdhA xanthine dehydrogenase, small subunit (NCBI) 145, 291
RSP_3555 xdhB xanthine dehydrogenase, large subunit (NCBI) 145, 291
RSP_3556 xdhC xanthine dehydrogenase chaperone/MPT insertion protein (NCBI) 145, 291
RSP_3557 RSP_3557 ABC transporter, fused ATPase domains (NCBI) 145, 291
RSP_3558 RSP_3558 ABC transporter, inner membrane subunit (NCBI) 145, 291
RSP_3559 RSP_3559 ABC transporter, inner membrane subunit (NCBI) 145, 291
RSP_3560 RSP_3560 Possible ABC transporter, periplasmic binding protein (NCBI) 145, 291
RSP_4047 pdhAa Pyruvate dehydrogenase E1 component, alpha subunit (NCBI) 25, 195
RSP_4049 pdhAb Pyruvate dehydrogenase E1 component, beta subunit (NCBI) 25, 291
RSP_4050 pdhB Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex (NCBI) 25, 291
RSP_6136 RSP_6136 hypothetical protein (NCBI) 243, 291
RSP_6192 RSP_6192 hypothetical protein (NCBI) 194, 291
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_4049
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend