Organism : Synechococcus elongatus PCC 7942 | Module List :
Synpcc7942_1792

porphobilinogen synthase (EC 4.2.1.24) (IMGterm)

CircVis
Functional Annotations (7)
Function System
Delta-aminolevulinic acid dehydratase cog/ cog
porphobilinogen synthase activity go/ molecular_function
tetrapyrrole biosynthetic process go/ biological_process
metal ion binding go/ molecular_function
Porphyrin and chlorophyll metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for Synpcc7942_1792
(Mouseover regulator name to see its description)

Warning: No Regulators were found for Synpcc7942_1792!

Warning: Synpcc7942_1792 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 6 motifs predicted.

Motif Table (6)
Motif Id e-value Consensus Motif Logo
1820 1.10e-01 atCttaAgaAagtcTaAAg
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1821 8.20e+03 TaaAaTtGttAaAAa
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1822 1.20e+04 TgcaGCAAtgaaTCT
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1892 3.40e-01 CaGacttTCcGCtaCtcTggCTT
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1893 4.70e+02 cAcAGC
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1894 9.20e+02 cAatCGtCAg
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for Synpcc7942_1792

Synpcc7942_1792 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Delta-aminolevulinic acid dehydratase cog/ cog
porphobilinogen synthase activity go/ molecular_function
tetrapyrrole biosynthetic process go/ biological_process
metal ion binding go/ molecular_function
Porphyrin and chlorophyll metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
Module neighborhood information for Synpcc7942_1792

Synpcc7942_1792 has total of 37 gene neighbors in modules 100, 125
Gene neighbors (37)
Gene Common Name Description Module membership
Synpcc7942_0214 hypothetical protein 100, 105
Synpcc7942_0268 hypothetical protein 41, 100
Synpcc7942_0285 carbon dioxide concentrating mechanism protein CcmK 125, 157
Synpcc7942_0293 hypothetical protein 125, 161
Synpcc7942_0377 hypothetical protein 100, 105
Synpcc7942_0439 magnesium protoporphyrin O-methyltransferase (EC:2.1.1.11) 125, 157
Synpcc7942_0441 5-(carboxyamino)imidazole ribonucleotide mutase (IMGterm) 125, 138
Synpcc7942_0469 phosphoglycerate mutase (EC 5.4.2.1) (IMGterm) 100, 105
Synpcc7942_0479 GTP-binding protein LepA (IMGterm) 100, 118
Synpcc7942_0533 hypothetical protein 72, 125
Synpcc7942_0534 glutamine--fructose-6-phosphate transaminase (IMGterm) 109, 125
Synpcc7942_0645 "glutamate-1-semialdehyde 2,1-aminomutase (EC 5.4.3.8) (IMGterm)" 100, 105
Synpcc7942_0657 hypothetical protein 100, 105
Synpcc7942_0674 coproporphyrinogen oxidase (EC 1.3.3.3) (IMGterm) 100, 105
Synpcc7942_0855 response regulator receiver domain protein (CheY-like) 100, 105
Synpcc7942_0856 response regulator receiver domain protein (CheY-like) 100, 105
Synpcc7942_0857 CheW protein (IMGterm) 100, 105
Synpcc7942_0858 methyl-accepting chemotaxis sensory transducer with phytochrome sensor (IMGterm) 100, 105
Synpcc7942_0859 CheA signal transduction histidine kinase (IMGterm) 100, 105
Synpcc7942_0860 CheW protein (IMGterm) 38, 100
Synpcc7942_1117 hypothetical protein 125, 170
Synpcc7942_1126 ABC transporter permease protein 72, 125
Synpcc7942_1188 Ap-4-A phosphorylase II-like protein 4, 100
Synpcc7942_1254 cyclic nucleotide-binding domain (cNMP-BD) protein 100, 105
Synpcc7942_1329 hypothetical protein 125, 184
Synpcc7942_1330 hypothetical protein 81, 125
Synpcc7942_1514 "Pseudouridine synthase, Rsu (EC:5.4.99.12)" 100, 110
Synpcc7942_1525 GTP-binding protein TypA 100, 165
Synpcc7942_1534 hypothetical protein 100, 167
Synpcc7942_1659 nitroreductase 125, 165
Synpcc7942_1695 hypothetical protein 125, 184
Synpcc7942_1743 NADH dehydrogenase subunit D (EC 1.6.5.3) (IMGterm) 81, 125
Synpcc7942_1781 molybdopterin synthase subunit MoaD (IMGterm) 3, 125
Synpcc7942_1792 porphobilinogen synthase (EC 4.2.1.24) (IMGterm) 100, 125
Synpcc7942_1898 "3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33) (IMGterm)" 100, 104
Synpcc7942_2159 hypothetical protein 125, 161
Synpcc7942_2161 hypothetical protein 125, 170
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for Synpcc7942_1792
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend