Organism : Bacillus cereus ATCC14579 | Module List :
BC4694

hypothetical protein (NCBI ptt file)

CircVis
Functional Annotations (5)
Function System
Predicted small secreted protein cog/ cog
extracellular region go/ cellular_component
proteolysis go/ biological_process
metallopeptidase activity go/ molecular_function
zinc ion binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for BC4694
(Mouseover regulator name to see its description)

BC4694 is regulated by 24 influences and regulates 0 modules.
Regulators for BC4694 (24)
Regulator Module Operator
BC0116 466 tf
BC3356 466 tf
BC3982 466 tf
BC4091 466 tf
BC4204 466 tf
BC4316 466 tf
BC4499 466 tf
BC5339 466 tf
BC5340 466 tf
BC5368 466 tf
BC0051 275 tf
BC0116 275 tf
BC1282 275 tf
BC2166 275 tf
BC3814 275 tf
BC3868 275 tf
BC3976 275 tf
BC4170 275 tf
BC4204 275 tf
BC4289 275 tf
BC4316 275 tf
BC5332 275 tf
BC5339 275 tf
BC5483 275 tf

Warning: BC4694 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
4466 3.60e+02 gAgGTGaaA
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4467 5.70e+03 CCctTGTTGGTAAG
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4842 1.10e-01 AcaatataAA.AAAGgG.cg
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4843 6.70e+01 AGG.cGgTcAa
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for BC4694

BC4694 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Predicted small secreted protein cog/ cog
extracellular region go/ cellular_component
proteolysis go/ biological_process
metallopeptidase activity go/ molecular_function
zinc ion binding go/ molecular_function
Module neighborhood information for BC4694

BC4694 has total of 23 gene neighbors in modules 275, 466
Gene neighbors (23)
Gene Common Name Description Module membership
BC0065 BC0065 Cell division protein DIVIC (NCBI ptt file) 41, 275
BC0066 BC0066 S1-type RNA-binding domain (NCBI ptt file) 275, 350
BC0115 BC0115 Protein translocase subunit SecE (NCBI ptt file) 231, 466
BC0504 BC0504 A/G-specific adenine DNA glycosylase (NCBI ptt file) 192, 466
BC1350 BC1350 hypothetical protein (NCBI ptt file) 390, 466
BC1351 BC1351 hypothetical protein (NCBI ptt file) 242, 466
BC3663 BC3663 Transporter, Drug/Metabolite Exporter family (NCBI ptt file) 37, 466
BC3796 BC3796 Zn-dependent hydrolase (NCBI ptt file) 319, 466
BC3868 BC3868 DNA-directed RNA polymerase omega chain (NCBI ptt file) 275, 479
BC3871 BC3871 Protein yicC (NCBI ptt file) 142, 275
BC3917 BC3917 Cell division protein ftsL (NCBI ptt file) 228, 275
BC4203 BC4203 Phosphoglycolate phosphatase (NCBI ptt file) 37, 466
BC4204 BC4204 Manganese transport transcription regulator (NCBI ptt file) 81, 466
BC4333 BC4333 putative lipase (NCBI ptt file) 275, 426
BC4371 BC4371 hypothetical protein (NCBI ptt file) 81, 466
BC4415 BC4415 Holliday junction DNA helicase ruvA (NCBI ptt file) 390, 466
BC4554 BC4554 Colicin V production protein (NCBI ptt file) 390, 466
BC4555 BC4555 hypothetical protein (NCBI ptt file) 390, 466
BC4556 BC4556 Ribonuclease HII (NCBI ptt file) 390, 466
BC4669 BC4669 Large-conductance mechanosensitive channel (NCBI ptt file) 37, 466
BC4694 BC4694 hypothetical protein (NCBI ptt file) 275, 466
BC5232 BC5232 Phosphoglycerol transferase (NCBI ptt file) 319, 466
BC5483 BC5483 Stage 0 sporulation protein J (NCBI ptt file) 126, 275
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for BC4694
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend