Organism : Campylobacter jejuni | Module List :
Cj0531 icd

isocitrate dehydrogenase (NCBI ptt file)

CircVis
Functional Annotations (10)
Function System
Monomeric isocitrate dehydrogenase cog/ cog
isocitrate dehydrogenase (NADP+) activity go/ molecular_function
tricarboxylic acid cycle go/ biological_process
Citrate cycle (TCA cycle) kegg/ kegg pathway
Glutathione metabolism kegg/ kegg pathway
Carbon fixation pathways in prokaryotes kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
monomer_idh tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for Cj0531
(Mouseover regulator name to see its description)

Cj0531 is regulated by 7 influences and regulates 0 modules.
Regulators for Cj0531 icd (7)
Regulator Module Operator
Cj0287c 74 tf
Cj0368c 74 tf
Cj0400 74 tf
Cj1533c 74 tf
Cj0287c 126 tf
Cj0368c 126 tf
Cj1533c 126 tf

Warning: Cj0531 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7530 2.90e+04 CTaGAGTGTc
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7531 3.40e+04 CAACTTCC
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7634 2.50e+00 AAGGAggAA
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7635 8.90e+03 TCAGcG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for Cj0531

Cj0531 is enriched for 10 functions in 3 categories.
Enrichment Table (10)
Function System
Monomeric isocitrate dehydrogenase cog/ cog
isocitrate dehydrogenase (NADP+) activity go/ molecular_function
tricarboxylic acid cycle go/ biological_process
Citrate cycle (TCA cycle) kegg/ kegg pathway
Glutathione metabolism kegg/ kegg pathway
Carbon fixation pathways in prokaryotes kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
monomer_idh tigr/ tigrfam
Module neighborhood information for Cj0531

Cj0531 has total of 35 gene neighbors in modules 74, 126
Gene neighbors (35)
Gene Common Name Description Module membership
Cj0002 dnaN DNA polymerase III, beta chain (NCBI ptt file) 65, 74
Cj0073c Cj0073c hypothetical protein Cj0073c (NCBI ptt file) 119, 126
Cj0074c Cj0074c putative iron-sulfur protein (NCBI ptt file) 119, 126
Cj0075c Cj0075c putative oxidoreductase iron-sulfur subunit (NCBI ptt file) 119, 126
Cj0076c lctP L-lactate permease (NCBI ptt file) 119, 126
Cj0193c tig trigger factor (peptidyl-prolyl cis /trans isomerase, chaperone) (NCBI ptt file) 74, 82
Cj0331c Cj0331c hypothetical protein Cj0331c (NCBI ptt file) 74, 120
Cj0332c ndk nucleoside diphosphate kinase (NCBI ptt file) 74, 170
Cj0337c motA putative flagellar motor proton channel (NCBI ptt file) 74, 142
Cj0360 Cj0360 putative phospho-sugar mutase (NCBI ptt file) 74, 102
Cj0408 frdC fumarate reductase cytochrome B subunit (NCBI ptt file) 20, 126
Cj0409 frdA fumarate reductase flavoprotein subunit (NCBI ptt file) 74, 126
Cj0441 acpP acyl carrier protein (NCBI ptt file) 74, 102
Cj0531 icd isocitrate dehydrogenase (NCBI ptt file) 74, 126
Cj0533 sucC succinyl-coA synthetase beta chain (NCBI ptt file) 119, 126
Cj0534 sucD succinyl-coA synthetase alpha chain (NCBI ptt file) 74, 126
Cj0535 oorD OORD subunit of 2-oxoglutarate:acceptor oxidoreductase (NCBI ptt file) 87, 126
Cj0536 oorA OORA subunit of 2-oxoglutarate:acceptor oxidoreductase (NCBI ptt file) 74, 126
Cj0538 oorC OORC subunit of 2-oxoglutarate:acceptor oxidoreductase (NCBI ptt file) 74, 102
Cj0780 napA periplasmic nitrate reductase (NCBI ptt file) 87, 126
Cj0833c Cj0833c oxidoreductase (NCBI ptt file) 74, 126
Cj0835c acnB aconitate hydratase (NCBI ptt file) 119, 126
Cj0852c Cj0852c putative integral membrane protein (NCBI ptt file) 74, 120
Cj1022c Cj1022c putative integral membrane protein (NCBI ptt file) 74, 95
Cj1058c guaB inosine-5'-monophosphate dehydrogenase (NCBI ptt file) 10, 74
Cj1110c Cj1110c putative MCP-type signal transduction protein (NCBI ptt file) 74, 102
Cj1265c hydC Ni/Fe-hydrogenase B-type cytochrome subunit (NCBI ptt file) 74, 170
Cj1266c hydB Ni/Fe-hydrogenase large subunit (NCBI ptt file) 10, 74
Cj1279c Cj1279c putative fibronectin domain-containing lipoprotein (NCBI ptt file) 53, 74
Cj1487c ccoP cb-type cytochrome C oxidase subunit III (NCBI ptt file) 74, 87
Cj1488c ccoQ cb-type cytochrome C oxidase subunit IV (NCBI ptt file) 74, 87
Cj1489c ccoO cb-type cytochrome C oxidase subunit II (NCBI ptt file) 20, 126
Cj1490c ccoN cb-type cytochrome C oxidase subunit I (NCBI ptt file) 74, 126
Cj1624c sdaA L-serine dehydratase (NCBI ptt file) 74, 126
Cj1625c sdaC serine transporter (NCBI ptt file) 74, 126
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for Cj0531
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend