Organism : Campylobacter jejuni | Module List :
Cj0953c purH

phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase (NCBI ptt file)

CircVis
Functional Annotations (9)
Function System
AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful) cog/ cog
IMP cyclohydrolase activity go/ molecular_function
phosphoribosylaminoimidazolecarboxamide formyltransferase activity go/ molecular_function
purine nucleotide biosynthetic process go/ biological_process
Purine metabolism kegg/ kegg pathway
One carbon pool by folate kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
purH tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for Cj0953c
(Mouseover regulator name to see its description)

Warning: No Regulators were found for Cj0953c!

Warning: Cj0953c Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7420 4.00e+02 ttcCTaAA
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7421 5.70e+03 GAACTTATCAGCC
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7590 1.10e+02 AaaaGaCttTTtT.gc.GttT.aA
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7591 4.10e+02 GaGaAttgGG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for Cj0953c

Cj0953c is enriched for 9 functions in 3 categories.
Enrichment Table (9)
Function System
AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful) cog/ cog
IMP cyclohydrolase activity go/ molecular_function
phosphoribosylaminoimidazolecarboxamide formyltransferase activity go/ molecular_function
purine nucleotide biosynthetic process go/ biological_process
Purine metabolism kegg/ kegg pathway
One carbon pool by folate kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
purH tigr/ tigrfam
Module neighborhood information for Cj0953c

Cj0953c has total of 51 gene neighbors in modules 19, 104
Gene neighbors (51)
Gene Common Name Description Module membership
Cj0036 Cj0036 hypothetical protein Cj0036 (NCBI ptt file) 19, 24
Cj0041 Cj0041 hypothetical protein Cj0041 (NCBI ptt file) 19, 36
Cj0067 Cj0067 hypothetical protein Cj0067 (NCBI ptt file) 98, 104
Cj0167c Cj0167c putative integral membrane protein (NCBI ptt file) 96, 104
Cj0204 Cj0204 putative integral membrane protein (NCBI ptt file) 104, 156
Cj0224 argC N-acetyl-gamma-glutamyl-phosphate reductase (NCBI ptt file) 104, 156
Cj0225 Cj0225 putative acetyltransferase (NCBI ptt file) 104, 109
Cj0226 argB acetylglutamate kinase (NCBI ptt file) 96, 104
Cj0227 argD acetylornithine aminotransferase (NCBI ptt file) 96, 104
Cj0252 moaC molybdenum cofactor biosynthesis protein C (NCBI ptt file) 19, 81
Cj0257 dgkA diacylglycerol kinase (NCBI ptt file) 24, 104
Cj0299 Cj0299 putative periplasmic beta-lactamase (NCBI ptt file) 19, 62
Cj0306c bioF 8-amino-7-oxononanoate synthase (NCBI ptt file) 96, 104
Cj0313 Cj0313 putative integral membrane protein (NCBI ptt file) 24, 104
Cj0328c fabH 3-oxoacyl-[acyl-carrier-protein] synthase (NCBI ptt file) 104, 151
Cj0340 Cj0340 putative nucleoside hydrolase (NCBI ptt file) 19, 37
Cj0345 trpE putative anthranilate synthase component I (NCBI ptt file) 104, 117
Cj0346 trpD anthranilate synthase component II (NCBI ptt file) 104, 117
Cj0347 trpF N-(5'-phosphoribosyl)anthranilate isomerase (NCBI ptt file) 88, 104
Cj0349 trpA tryptophan synthase alpha chain (NCBI ptt file) 104, 109
Cj0432c murD UDP-N-acetylmuramoylalanine--D-glutamate ligase (NCBI ptt file) 76, 104
Cj0589 ribF putative riboflavin kinase /FMN adenylyltransferase (NCBI ptt file) 104, 145
Cj0646 Cj0646 putative lipoprotein (NCBI ptt file) 19, 63
Cj0791c Cj0791c putative aminotransferase (NCBI ptt file) 19, 24
Cj0800c Cj0800c hypothetical protein Cj0800c (NCBI ptt file) 6, 19
Cj0808c Cj0808c small hydrophobic protein (NCBI ptt file) 6, 104
Cj0820c fliP flagellar biosynthesis protein (NCBI ptt file) 104, 160
Cj0821 glmU UDP-N-acetylglucosamine pyrophosphorylase (NCBI ptt file) 104, 110
Cj0822 dfp DNA /pantothenate metabolism flavoprotein (NCBI ptt file) 19, 167
Cj0823 Cj0823 hypothetical protein Cj0823 (NCBI ptt file) 104, 164
Cj0824 uppS putative undecaprenyl diphosphate synthase (NCBI ptt file) 104, 158
Cj0825 Cj0825 putative processing peptidase (NCBI ptt file) 104, 141
Cj0826 Cj0826 putative integral membrane protein (NCBI ptt file) 104, 150
Cj0827 truA putative tRNA pseudouridine synthase (NCBI ptt file) 89, 104
Cj0953c purH phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase (NCBI ptt file) 19, 104
Cj0955c purL phosphoribosylformylglycinamidine synthase (NCBI ptt file) 19, 26
Cj0964 Cj0964 putative periplasmic protein (NCBI ptt file) 19, 55
Cj1015c livG branched-chain amino-acid ABC transport system ATP-binding protein (NCBI ptt file) 104, 151
Cj1016c livM putative branched-chain amino-acid ABC transport system permease protein (NCBI ptt file) 104, 128
Cj1028c Cj1028c possible purine/pyrimidine phosphoribosyltransferase (NCBI ptt file) 81, 104
Cj1157 dnaX putative DNA polymerase III subunit gamma (NCBI ptt file) 7, 19
Cj1241 Cj1241 putative transmembrane transport protein (NCBI ptt file) 19, 167
Cj1376 Cj1376 putative periplasmic protein (NCBI ptt file) 19, 144
Cj1433c Cj1433c hypothetical protein Cj1433c (NCBI ptt file) 19, 36
Cj1472c Cj1472c putative membrane protein (NCBI ptt file) 7, 104
Cj1562 Cj1562 hypothetical protein Cj1562 (NCBI ptt file) 19, 63
Cj1601 hisA phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (NCBI ptt file) 19, 109
Cj1616 chuC putative haemin uptake system ATP-binding protein (NCBI ptt file) 2, 104
Cj1651c map methionine aminopeptidase (NCBI ptt file) 19, 144
VIMSS46212 VIMSS46212 None 6, 19
VIMSS46406 VIMSS46406 None 19, 35
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

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Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
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Comments for Cj0953c
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend