Organism : Campylobacter jejuni | Module List :
Cj1201 metE

5-methyltetrahydropteroyltriglutamate--homocystei methyltransferase (NCBI ptt file)

CircVis
Functional Annotations (8)
Function System
Methionine synthase II (cobalamin-independent) cog/ cog
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity go/ molecular_function
zinc ion binding go/ molecular_function
methionine biosynthetic process go/ biological_process
Cysteine and methionine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
met_syn_B12ind tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for Cj1201
(Mouseover regulator name to see its description)

Cj1201 is regulated by 6 influences and regulates 0 modules.
Regulators for Cj1201 metE (6)
Regulator Module Operator
Cj0287c 105 tf
Cj0382c 105 tf
Cj0400 105 tf
Cj0480c 105 tf
Cj0670 105 tf
Cj1230 105 tf

Warning: Cj1201 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7432 2.80e+03 GCGTCC
Loader icon
7433 4.60e+03 GCTGcG
Loader icon
7592 8.10e+00 GCg.attctC.ttaG
Loader icon
7593 5.50e+01 gTAGCTcagcaGG
Loader icon
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for Cj1201

Cj1201 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
Methionine synthase II (cobalamin-independent) cog/ cog
5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity go/ molecular_function
zinc ion binding go/ molecular_function
methionine biosynthetic process go/ biological_process
Cysteine and methionine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
met_syn_B12ind tigr/ tigrfam
Module neighborhood information for Cj1201

Cj1201 has total of 36 gene neighbors in modules 25, 105
Gene neighbors (36)
Gene Common Name Description Module membership
Cj0038c Cj0038c putative membrane protein (NCBI ptt file) 105, 152
Cj0122 Cj0122 hypothetical protein Cj0122 (NCBI ptt file) 22, 105
Cj0190c Cj0190c hypothetical protein Cj0190c (NCBI ptt file) 105, 152
Cj0300c modC putative molybdenum transport ATP-binding protein (NCBI ptt file) 101, 105
Cj0318 fliF flagellar M-ring protein (NCBI ptt file) 25, 42
Cj0342c uvrA excinuclease ABC subunit A (NCBI ptt file) 12, 105
Cj0430 Cj0430 putative integral membrane protein (NCBI ptt file) 22, 105
Cj0551 efp elongation factor P (NCBI ptt file) 25, 46
Cj0593c Cj0593c putative integral membrane protein (NCBI ptt file) 25, 89
Cj0598 Cj0598 putative membrane protein (NCBI ptt file) 25, 58
Cj0649 Cj0649 hypothetical protein Cj0649 (NCBI ptt file) 45, 105
Cj0804 pyrD dihydroorotate dehydrogenase (NCBI ptt file) 25, 29
Cj0819 Cj0819 small hydrophobic protein (NCBI ptt file) 25, 131
Cj0837c Cj0837c hypothetical protein Cj0837c (NCBI ptt file) 53, 105
Cj0841c Cj0841c putative ATP/GTP binding protein (NCBI ptt file) 25, 34
Cj0881c Cj0881c hypothetical protein Cj0881c (NCBI ptt file) 25, 53
Cj0970 Cj0970 hypothetical protein Cj0970 (NCBI ptt file) 105, 108
Cj1051c Cj1051c restriction modification enzyme (NCBI ptt file) 58, 105
Cj1056c Cj1056c hypothetical protein Cj1056c (NCBI ptt file) 25, 131
Cj1057c Cj1057c putative coiled-coil protein (NCBI ptt file) 25, 26
Cj1102 truB tRNA pseudouridine synthase B (NCBI ptt file) 22, 105
Cj1201 metE 5-methyltetrahydropteroyltriglutamate--homocystei methyltransferase (NCBI ptt file) 25, 105
Cj1272c spoT putative guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (NCBI ptt file) 25, 51
Cj1321 Cj1321 putative transferase (NCBI ptt file) 101, 105
Cj1410c Cj1410c putative membrane protein (NCBI ptt file) 25, 53
Cj1618c Cj1618c hypothetical protein Cj1618c (NCBI ptt file) 105, 108
Cj1621 Cj1621 putative periplasmic protein (NCBI ptt file) 25, 152
Cj1649 Cj1649 putative lipoprotein (NCBI ptt file) 101, 105
Cjp10 tRNA-Arg tRNA-Arg (NCBI) 85, 105
Cjp13 tRNA-Gln tRNA-Gln (NCBI) 5, 105
Cjp15 tRNA-Ile tRNA-Ile (NCBI) 100, 105
Cjp16 tRNALeu tRNA-Leu (RefSeq) 4, 105
Cjr03 rrf 5S ribosomal RNA (NCBI) 5, 105
Cjr05 rrl 23S ribosomal RNA (NCBI) 5, 105
Cjs01 Cjs01 None 5, 105
Cjt4 tRNA-Arg tRNA-Arg (NCBI) 30, 105
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for Cj1201
Please add your comments for this gene by using the form below. Your comments will be publicly available.

comments powered by Disqus

Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend