Organism : Geobacter sulfurreducens | Module List :
GSU0260

universal stress protein family (VIMSS)

CircVis
Functional Annotations (2)
Function System
Universal stress protein UspA and related nucleotide-binding proteins cog/ cog
response to stress go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for GSU0260
(Mouseover regulator name to see its description)

GSU0260 is regulated by 15 influences and regulates 0 modules.
Regulators for GSU0260 (15)
Regulator Module Operator
GSU0473 178 tf
GSU1410 178 tf
GSU1522 178 tf
GSU1626 178 tf
GSU1727 178 tf
GSU2926 178 tf
GSU3217 178 tf
GSU3229 178 tf
GSU1320 14 tf
GSU1626 14 tf
GSU2581 14 tf
GSU2587 14 tf
GSU2716 14 tf
GSU2753 14 tf
GSU3217 14 tf

Warning: GSU0260 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2188 9.80e+03 TattaTtataaTgtaTACatT
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2189 4.30e+02 GcGgTgcTcctGGTG.TgGcG
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2516 3.20e+01 GTATcTTTaCtAAGtAaccgATA
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2517 5.60e+03 tgCaa.accG.t.AtTGAcA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for GSU0260

GSU0260 is enriched for 2 functions in 3 categories.
Enrichment Table (2)
Function System
Universal stress protein UspA and related nucleotide-binding proteins cog/ cog
response to stress go/ biological_process
Module neighborhood information for GSU0260

GSU0260 has total of 39 gene neighbors in modules 14, 178
Gene neighbors (39)
Gene Common Name Description Module membership
GSU0121 GSU0121 nickel-iron hydrogenase, b-type cytochrome subunit (VIMSS) 14, 266
GSU0202 GSU0202 conserved hypothetical protein (NCBI) 178, 339
GSU0260 GSU0260 universal stress protein family (VIMSS) 14, 178
GSU0261 GSU0261 sodium/hydrogen exchanger family protein (VIMSS) 32, 178
GSU0394 GSU0394 AcrB/AcrD/AcrF family protein (VIMSS) 14, 229
GSU0411 fliG flagellar motor switch protein FliG (NCBI) 14, 186
GSU0450 GSU0450 conserved hypothetical protein (VIMSS) 178, 259
GSU0480 GSU0480 NifU-like domain protein (NCBI) 130, 178
GSU0571 folA dihydrofolate reductase (NCBI) 14, 281
GSU0659 GSU0659 polysaccharide deacetylase domain protein (VIMSS) 14, 97
GSU0710 GSU0710 hypothetical protein (VIMSS) 178, 288
GSU0728 GSU0728 conserved hypothetical protein (VIMSS) 178, 261
GSU0745 GSU0745 NAD-dependent dehydrogenase subunit (NCBI) 178, 238
GSU0971 prfA peptidyl-tRNA hydrolase domain protein (NCBI) 153, 178
GSU0974 GSU0974 hypothetical protein (VIMSS) 178, 250
GSU1031 GSU1031 hypothetical protein (VIMSS) 14, 337
GSU1064 GSU1064 hypothetical protein (VIMSS) 14, 220
GSU1154 GSU1154 surface repeat protein, putative (NCBI) 14, 268
GSU1204 GSU1204 sodium/hydrogen exchanger family/TrkA domain protein (VIMSS) 14, 282
GSU1300 GSU1300 methyl-accepting chemotaxis protein (VIMSS) 14, 78
GSU1320 GSU1320 sigma-54 dependent DNA-binding response regulator (VIMSS) 14, 174
GSU1371 GSU1371 oxidoreductase, FAD/FMN-binding (NCBI) 14, 107
GSU1376 GSU1376 hypothetical protein (VIMSS) 178, 218
GSU1482 GSU1482 outer membrane efflux protein (VIMSS) 14, 295
GSU1948 GSU1948 hypothetical protein (VIMSS) 14, 292
GSU2098 cooS carbon monoxide dehydrogenase subunit (NCBI) 1, 178
GSU2487 arcC carbamate kinase (NCBI) 178, 220
GSU2494 GSU2494 cytochrome c family protein (VIMSS) 46, 178
GSU2581 GSU2581 sigma-54 dependent transcriptional regulator (VIMSS) 14, 186
GSU2907 GSU2907 membrane protein, putative (VIMSS) 14, 109
GSU2914 GSU2914 NHL repeat protein (NCBI) 178, 259
GSU2948 GSU2948 membrane protein, putative (NCBI) 103, 178
GSU2959 GSU2959 membrane protein, putative (NCBI) 14, 213
GSU2981 GSU2981 TonB-dependent receptor, putative (VIMSS) 14, 148
GSU3017 GSU3017 conserved domain protein (VIMSS) 178, 244
GSU3189 GSU3189 hypothetical protein (VIMSS) 178, 252
GSU3395 putA proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase (NCBI) 178, 294
GSU3396 GSU3396 transcriptional regulator, GntR family (VIMSS) 178, 295
GSU3419 GSU3419 sensor histidine kinase (VIMSS) 14, 274
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for GSU0260
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend