Organism : Geobacter sulfurreducens | Module List :
GSU0547 mutS2

MutS2 family protein (NCBI)

CircVis
Functional Annotations (7)
Function System
Mismatch repair ATPase (MutS family) cog/ cog
ATP binding go/ molecular_function
cytoplasm go/ cellular_component
mismatch repair go/ biological_process
mismatched DNA binding go/ molecular_function
Mismatch repair kegg/ kegg pathway
mutS2 tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for GSU0547
(Mouseover regulator name to see its description)

GSU0547 is regulated by 20 influences and regulates 0 modules.
Regulators for GSU0547 mutS2 (20)
Regulator Module Operator
GSU0063 287 tf
GSU0735 287 tf
GSU0812 287 tf
GSU2041 287 tf
GSU2581 287 tf
GSU2587 287 tf
GSU2716 287 tf
GSU2779 287 tf
GSU2787 287 tf
GSU2868 287 tf
GSU3217 287 tf
GSU3363 287 tf
GSU0266 252 tf
GSU0735 252 tf
GSU1115 252 tf
GSU2581 252 tf
GSU2831 252 tf
GSU2868 252 tf
GSU3217 252 tf
GSU3387 252 tf

Warning: GSU0547 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2664 8.40e-01 tAaacACCaaaaccg.AtgaA
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2665 2.00e-01 cC..cCCgC.cagAa.g.cG
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2732 1.00e+01 gTAAAAat.aCCCacT
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2733 1.10e+03 tT.TTCcTCTagccTAacCaacCt
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for GSU0547

GSU0547 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Mismatch repair ATPase (MutS family) cog/ cog
ATP binding go/ molecular_function
cytoplasm go/ cellular_component
mismatch repair go/ biological_process
mismatched DNA binding go/ molecular_function
Mismatch repair kegg/ kegg pathway
mutS2 tigr/ tigrfam
Module neighborhood information for GSU0547

GSU0547 has total of 46 gene neighbors in modules 252, 287
Gene neighbors (46)
Gene Common Name Description Module membership
GSU0048 GSU0048 integrase (NCBI) 287, 300
GSU0123 GSU0123 nickel-dependent hydrogenase, small subunit (VIMSS) 96, 252
GSU0270 glmS glucosamine--fructose-6-phosphate aminotransferase (isomerizing) (NCBI) 285, 287
GSU0471 GSU0471 sensor histidine kinase (VIMSS) 252, 268
GSU0543 GSU0543 outer membrane lipoprotein, Slp family, putative (VIMSS) 229, 252
GSU0544 GSU0544 conserved hypothetical protein (NCBI) 229, 252
GSU0545 GSU0545 membrane protein, putative (VIMSS) 226, 252
GSU0546 GSU0546 peptidyl-tRNA hydrolase domain protein (NCBI) 69, 252
GSU0547 mutS2 MutS2 family protein (NCBI) 252, 287
GSU0549 GSU0549 conserved domain protein (NCBI) 122, 287
GSU0582 GSU0582 methyl-accepting chemotaxis protein (VIMSS) 281, 287
GSU0605 thiD thiamine-phosphate pyrophosphorylase/phosphomethylpyrimidine kinase (NCBI) 99, 287
GSU0791 GSU0791 conserved hypothetical protein (VIMSS) 107, 252
GSU0795 GSU0795 Rhodanese-like domain protein (VIMSS) 173, 252
GSU0901 fragment conserved domain protein (NCBI) 257, 287
GSU0916 GSU0916 methyl-accepting chemotaxis protein (VIMSS) 186, 287
GSU0967 GSU0967 membrane protein, putative (VIMSS) 32, 252
GSU1029 GSU1029 methyl-accepting chemotaxis protein (VIMSS) 63, 287
GSU1034 GSU1034 None 287, 300
GSU1101 GSU1101 sensory box histidine kinase (VIMSS) 252, 287
GSU1238 GSU1238 iron-sulfur cluster-binding protein (VIMSS) 72, 252
GSU1383 dnaQ exonuclease (NCBI) 287, 300
GSU1552 GSU1552 conserved hypothetical protein (VIMSS) 220, 287
GSU1567 htpX peptidase, M48 family (NCBI) 78, 287
GSU1742 GSU1742 Na/Pi-cotransporter family protein (VIMSS) 287, 293
GSU2094 GSU2094 response regulator (VIMSS) 1, 287
GSU2174 GSU2174 conserved hypothetical protein (VIMSS) 229, 252
GSU2300 GSU2300 hypothetical protein (VIMSS) 99, 287
GSU2441 GSU2441 conserved hypothetical protein (VIMSS) 287, 317
GSU2463 GSU2463 glycosyl transferase, group 1 family protein (NCBI) 165, 252
GSU2480 kdpA potassium-transporting ATPase, A subunit (NCBI) 96, 252
GSU2574 GSU2574 response regulator (VIMSS) 122, 287
GSU2594 GSU2594 ISGsu6, transposase OrfB (VIMSS) 287, 300
GSU2636 GSU2636 alpha-amylase family protein (VIMSS) 195, 252
GSU2659 GSU2659 hypothetical protein (VIMSS) 183, 287
GSU2661 GSU2661 conserved hypothetical protein (VIMSS) 1, 287
GSU2759 GSU2759 potassium efflux system protein (VIMSS) 183, 287
GSU2762 glpK glycerol kinase (NCBI) 4, 252
GSU2784 GSU2784 hypothetical protein (VIMSS) 6, 252
GSU2984 znuC permease component of zinc ABC transporter (Dmitry Rodionov) 96, 287
GSU3150 GSU3150 HPP family protein (NCBI) 26, 287
GSU3153 GSU3153 iron-sulfur cluster-binding protein (VIMSS) 252, 266
GSU3189 GSU3189 hypothetical protein (VIMSS) 178, 252
GSU3217 GSU3217 sigma-54 dependent DNA-binding response regulator (VIMSS) 92, 287
GSU3224 GSU3224 hypothetical protein (VIMSS) 287, 337
GSU3372 GSU3372 cardiolipin synthetase, putative (VIMSS) 287, 291
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for GSU0547
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend