Organism : Geobacter sulfurreducens | Module List :
GSU1659 hisS

histidyl-tRNA synthetase (NCBI)

CircVis
Functional Annotations (7)
Function System
Histidyl-tRNA synthetase cog/ cog
histidine-tRNA ligase activity go/ molecular_function
ATP binding go/ molecular_function
cytoplasm go/ cellular_component
histidyl-tRNA aminoacylation go/ biological_process
Aminoacyl-tRNA biosynthesis kegg/ kegg pathway
hisS tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for GSU1659
(Mouseover regulator name to see its description)

GSU1659 is regulated by 25 influences and regulates 0 modules.
Regulators for GSU1659 hisS (25)
Regulator Module Operator
GSU0018 276 tf
GSU0031 276 tf
GSU0372 276 tf
GSU1617 276 tf
GSU1692 276 tf
GSU2237 276 tf
GSU2262 276 tf
GSU2817 276 tf
GSU3298 276 tf
GSU3396 276 tf
GSU0013 208 tf
GSU0254 208 tf
GSU0732 208 tf
GSU0836 208 tf
GSU1115 208 tf
GSU1129 208 tf
GSU1320 208 tf
GSU1410 208 tf
GSU1522 208 tf
GSU1692 208 tf
GSU1727 208 tf
GSU1989 208 tf
GSU2523 208 tf
GSU2581 208 tf
GSU3324 208 tf

Warning: GSU1659 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2576 3.00e+02 TcATg.AaaAa
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2577 1.90e+02 cAAaaCCACgttC
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2712 2.40e+03 aAaATgtgaataT
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2713 3.00e+01 gCatTtT.atc
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for GSU1659

GSU1659 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Histidyl-tRNA synthetase cog/ cog
histidine-tRNA ligase activity go/ molecular_function
ATP binding go/ molecular_function
cytoplasm go/ cellular_component
histidyl-tRNA aminoacylation go/ biological_process
Aminoacyl-tRNA biosynthesis kegg/ kegg pathway
hisS tigr/ tigrfam
Module neighborhood information for GSU1659

GSU1659 has total of 47 gene neighbors in modules 208, 276
Gene neighbors (47)
Gene Common Name Description Module membership
GSU0008 GSU0008 sensory box histidine kinase/response regulator (VIMSS) 190, 208
GSU0118 GSU0118 hypothetical protein (VIMSS) 30, 276
GSU0531 dapF diaminopimelate epimerase (NCBI) 80, 276
GSU0612 ppcA cytochrome c3 (NCBI) 162, 276
GSU0735 GSU0735 PTS system IIA component, fructose subfamily (VIMSS) 238, 276
GSU0737 GSU0737 hypothetical protein (NCBI) 265, 276
GSU0813 GSU0813 conserved hypothetical protein (VIMSS) 30, 276
GSU0836 GSU0836 nucleoside diphosphate kinase regulator protein, putative (VIMSS) 208, 317
GSU0839 GSU0839 hypothetical protein (VIMSS) 155, 208
GSU0847 GSU0847 rubredoxin (VIMSS) 208, 289
GSU1011 GSU1011 hypothetical protein (VIMSS) 208, 219
GSU1077 ruvB Holliday junction DNA helicase RuvB (NCBI) 208, 260
GSU1118 GSU1118 universal stress protein family (VIMSS) 264, 276
GSU1133 GSU1133 hypothetical protein (VIMSS) 81, 276
GSU1175 tgt-1 queuine tRNA-ribosyltransferase (NCBI) 276, 308
GSU1259 GSU1259 hypothetical protein (VIMSS) 176, 208
GSU1274 GSU1274 radical SAM domain protein (NCBI) 126, 208
GSU1321 GSU1321 thioredoxin family protein (VIMSS) 52, 208
GSU1396 GSU1396 hypothetical protein (VIMSS) 65, 208
GSU1397 GSU1397 cytochrome c family protein, putative (NCBI) 65, 208
GSU1429 GSU1429 membrane protein, putative (NCBI) 190, 208
GSU1492 pilT-4 twitching motility protein PilT (NCBI) 75, 208
GSU1565 GSU1565 hypothetical protein (VIMSS) 276, 300
GSU1652 GSU1652 hypothetical protein (VIMSS) 106, 276
GSU1659 hisS histidyl-tRNA synthetase (NCBI) 208, 276
GSU1698 GSU1698 TPR domain protein (VIMSS) 39, 276
GSU1757 rimI ribosomal-protein-alanine acetyltransferase (VIMSS) 136, 208
GSU1821 GSU1821 N-acetylmuramoyl-L-alanine amidase, family 3 (VIMSS) 42, 208
GSU1843 GSU1843 metallo-beta-lactamase family protein (NCBI) 190, 208
GSU1910 ilvN acetolactate synthase, small subunit (NCBI) 88, 276
GSU1911 ilvB acetolactate synthase, large subunit, biosynthetic type (NCBI) 228, 276
GSU1912 ilvD dihydroxy-acid dehydratase (NCBI) 39, 276
GSU1915 dxr 1-deoxy-D-xylulose 5-phosphate reductoisomerase (NCBI) 208, 333
GSU1922 GSU1922 membrane protein, putative (VIMSS) 190, 208
GSU2099 GSU2099 sensory box protein, putative (VIMSS) 8, 276
GSU2104 GSU2104 lipoprotein, putative (VIMSS) 106, 276
GSU2241 GSU2241 capsular polysaccharide biosynthesis protein I (VIMSS) 197, 208
GSU2426 GSU2426 conserved hypothetical protein (NCBI) 208, 225
GSU2537 speA biosynthetic arginine decarboxylase (NCBI) 197, 208
GSU2564 GSU2564 hypothetical protein (VIMSS) 44, 208
GSU2765 GSU2765 hypothetical protein (VIMSS) 208, 225
GSU2814 GSU2814 rubrerythrin (VIMSS) 208, 333
GSU3278 GSU3278 hypothetical protein (VIMSS) 169, 276
GSU3304 GSU3304 LamB porin family protein, putative (VIMSS) 30, 276
GSU3312 hemH ferrochelatase (NCBI) 30, 276
GSU3378 GSU3378 glutamate-ammonia ligase adenylyltransferase domain protein (NCBI) 111, 208
GSU3385 pckA phosphoenolpyruvate carboxykinase (NCBI) 137, 276
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for GSU1659
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend