Rv0022c WhiB family transcriptional regulator


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0022c whiB5 WhiB family transcriptional regulator CDS 27023 27442 - 420 139 TRUE

Rv0022c (WhiB family transcriptional regulator) is predicted to be co-regulated in modules bicluster_0211 with residual 0.50 and bicluster_0558 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.05 for bicluster_0211 and 0.00 and 0.69 for bicluster_0558 respectively.

These modules are enriched for following go terms: translation, non-membrane-bounded organelle, intracellular non-membrane-bounded organ..., organelle, intracellular organelle, cytoplasmic part response to stress, S-methyltransferase activity, O-acyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 10 of 53
Gene Target Differential Expression Distance Expression pvalue Type
Induced 18 2.58 1.26e-32 Primary.TSS
Serine hydroxymethyltransferase (EC
Induced -109 0.98 0.000722688 Primary.TSS
Partial REP13E12 repeat protein
No -42 -0.01 0.994074 CDS
No -123 -0.56 0.0268591 Primary.TSS
No 12 -0.45 0.0942454 CDS
Induced -35 0.81 0.000679322 Primary.TSS
Chaperone protein DnaK
No -17 -0.11 0.844548 Primary.TSS
PE_PGRS family protein
No -147 -0.35 0.671448 CDS
No -93 0.35 0.14891 CDS
Possible membrane protein
Repressed 53 -0.62 0.0138199 Antisense.TSS
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
DNA-binding response regulator TrcR
No 56 -0.09 0.885287 CDS
Product (LegacyBRC) Product (RefSeq)
Operon # Operon
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15607164 NP_214536.1 Run

cell wall

cell wall

The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426371 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426372 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426373 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv0022c_B389 UCSC Browser Tracks

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.580000 1.41

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH:
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 3.72 U
D3I 3 3 3.83 3.69 I
D3U 3 3 3.83 1.93 U
D5I 9 5 6.00 I
D5U 17 5 6.00 U
D7I 18 7 8.14 3.28 I
D7U 19 7 8.14 2.96 U
D14I 4 14 15.63 I
D14U 4 14 15.63 4.79 U
D17I 3 17 19.15 I
D17U 3 17 19.15 2.71 U
D21I 4 21 23.23 1.63 I
D21U 4 21 23.23 3.25 U
D24I 3 24 26.60 I
D24U 3 24 26.60 4.43 U
D28I 4 28 30.61 I
D28U 4 28 30.61 2.80 U