Rv0111 Transmembrane acyltransferase (EC:2.3.1.-)

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv0111 Transmembrane acyltransferase (EC:2.3.1.-) CDS 133950 136007 + 2 058 685 FALSE

Rv0111 (Transmembrane acyltransferase (EC:2.3.1.-)) is predicted to be co-regulated in modules bicluster_0215 with residual 0.50 and bicluster_0443 with residual 0.45.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 13.00 and 140.00 for bicluster_0215 and 0.00 and 0.40 for bicluster_0443 respectively.

These modules are enriched for following go terms: cellular biosynthetic process, organic substance biosynthetic process, cytoplasm, RNA binding.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-1.647 147 133845 133992 133950
Last update: 10/16/2017 - 17:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14709 MT0120 247
Product (LegacyBRC) Product (RefSeq)
POSSIBLE TRANSMEMBRANE ACYLTRANSFERASE transmembrane acyltransferase
Operon # Operon
74
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Tyrosine metabolism

41
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Naphthalene degradation

40
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Ethylbenzene degradation

11
Total items in this category:  

KEGG

Limonene and pinene degradation

65
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607253 NP_214625.1 Run
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.020000 3.87

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: