Rv0937c Ku domain protein

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0937c mku Ku domain protein CDS 1045199 1046020 - 822 273 FALSE

Rv0937c (Ku domain protein) is predicted to be co-regulated in modules bicluster_0388 with residual 0.53 and bicluster_0397 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.05 for bicluster_0388 and 0.00 and 0.00 for bicluster_0397 respectively.

These modules are enriched for following go terms: nitrogen compound metabolic process, carbon-oxygen lyase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
624
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Non-homologous end-joining

5
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608077 NP_215452.1 Run
GO:0003690

double-stranded DNA binding

double-stranded DNA binding

Details: 
Interacting selectively and non-covalently with double-stranded DNA.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0006303

double-strand break repair via nonhomologous end joining

double-strand break repair via nonhomologous end joining

Details: 
The repair of a double-strand break in DNA in which the two broken ends are rejoined with little or no sequence complementarity. Information at the DNA ends may be lost due to the modification of broken DNA ends.
GO Category: 
biological_process
2
Total items in this category:  
GO:0033202

DNA helicase complex

DNA helicase complex

Details: 
A protein complex that possesses DNA helicase activity.
GO Category: 
cellular_component
2
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.770000 1.55

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: