Rv1093 Serine hydroxymethyltransferase 1 (EC 2.1.2.1)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1093 glyA1 Serine hydroxymethyltransferase 1 (EC 2.1.2.1) CDS 1220574 1221854 + 1 281 426 FALSE

Rv1093 (Serine hydroxymethyltransferase 1 (EC 2.1.2.1)) is predicted to be co-regulated in modules bicluster_0367 with residual 0.59 and bicluster_0581 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0367 and 0.01 and 120.00 for bicluster_0581 respectively.

These modules are enriched for following go terms: protein folding, propionyl-CoA carboxylase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Serine hydroxymethyltransferase 1 serine hydroxymethyltransferase
Operon # Operon
738
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycine, serine and threonine metabolism

27
Total items in this category:  

KEGG

Cyanoamino acid metabolism

12
Total items in this category:  

KEGG

One carbon pool by folate

13
Total items in this category:  

KEGG

Methane metabolism

26
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116827 YP_177787.1 Run
GO:0004372

glycine hydroxymethyltransferase activity

glycine hydroxymethyltransferase activity

Details: 
Catalysis of the reaction: 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004372

glycine hydroxymethyltransferase activity

glycine hydroxymethyltransferase activity

Details: 
Catalysis of the reaction: 5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0006544

glycine metabolic process

glycine metabolic process

Details: 
The chemical reactions and pathways involving glycine, aminoethanoic acid.
GO Category: 
biological_process
1
Total items in this category:  
GO:0006563

L-serine metabolic process

L-serine metabolic process

Details: 
The chemical reactions and pathways involving L-serine, the L-enantiomer of serine, i.e. (2S)-2-amino-3-hydroxypropanoic acid.
GO Category: 
biological_process
3
Total items in this category:  
GO:0030170

pyridoxal phosphate binding

pyridoxal phosphate binding

Details: 
Interacting selectively and non-covalently with pyridoxal 5' phosphate, 3-hydroxy-5-(hydroxymethyl)-2-methyl4-pyridine carboxaldehyde 5' phosphate, the biologically active form of vitamin B6.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0042783

active evasion of host immune response

active evasion of host immune response

Details: 
Any mechanism of immune avoidance that directly affects the host immune system, e.g. blocking any stage in host MHC class I and II presentation. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
20
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: