Rv2601 Spermidine synthase (EC


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2601 speE Spermidine synthase (EC CDS 2928388 2929959 + 1 572 523 FALSE

Rv2601 (Spermidine synthase (EC is predicted to be co-regulated in modules bicluster_0191 with residual 0.46 and bicluster_0415 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.27 and 2.30 for bicluster_0191 and 37.00 and 3,500.00 for bicluster_0415 respectively.

These modules are enriched for following go terms: response to stress.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15068 MT2675 2621
Product (LegacyBRC) Product (RefSeq)
Probable spermidine synthase spermidine synthase
Operon # Operon
1706 -
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Cysteine and methionine metabolism

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Arginine and proline metabolism

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beta-Alanine metabolism

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Glutathione metabolism

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Metabolic pathways

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
57117003 YP_177892.1 Run

spermidine synthase activity

spermidine synthase activity

Catalysis of the reaction: S-adenosylmethioninamine + putrescine = 5'-methylthioadenosine + spermidine.
GO Category: 
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cell wall

cell wall

The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
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No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.140000 1.02

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: