Rv2988c 3-isopropylmalate dehydratase large subunit (EC


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2988c leuC 3-isopropylmalate dehydratase large subunit (EC CDS 3344654 3346075 - 1 422 473 FALSE

Rv2988c (3-isopropylmalate dehydratase large subunit (EC is predicted to be co-regulated in modules bicluster_0286 with residual 0.44 and bicluster_0344 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 4.30 and 5.80 for bicluster_0286 and 0.10 and 8.00 for bicluster_0344 respectively.

These modules are enriched for following go terms: branched-chain amino acid biosynthetic p..., branched-chain amino acid metabolic proc..., cytosol, cytosolic part .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
3-isopropylmalate dehydratase large subunit isopropylmalate isomerase large subunit
Operon # Operon
1950 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


3-isopropylmalate dehydratase Valine, leucine and isoleucine biosynthesis
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Valine, leucine and isoleucine biosynthesis

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C5-Branched dibasic acid metabolism

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Metabolic pathways

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Biosynthesis of secondary metabolites

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15610125 NP_217504.1 Run

3-isopropylmalate dehydratase activity

3-isopropylmalate dehydratase activity

Catalysis of the reaction: 3-isopropylmalate = 2-isopropylmaleate + H2O.
GO Category: 
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cell wall

cell wall

The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
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No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: