Rv3158 NADH-ubiquinone oxidoreductase chain N (EC

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3158 nuoN NADH-ubiquinone oxidoreductase chain N (EC CDS 3525790 3527385 + 1 596 531 FALSE

Rv3158 (NADH-ubiquinone oxidoreductase chain N (EC is predicted to be co-regulated in modules bicluster_0392 with residual 0.64 and bicluster_0541 with residual 0.44.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,800.00 and 3,000.00 for bicluster_0392 and 0.02 and 0.19 for bicluster_0541 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 14:56
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15702 MT3246 2798
Product (LegacyBRC) Product (RefSeq)
Rv3158 NADH dehydrogenase subunit N
Operon # Operon
2058 - - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


NADH dehydrogenase (ubiquinone) Oxidative phosphorylation
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Oxidative phosphorylation

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Metabolic pathways

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15610294 NP_217674.1 Run

NADH dehydrogenase (ubiquinone) activity

NADH dehydrogenase (ubiquinone) activity

Catalysis of the reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol.
GO Category: 
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extracellular region

extracellular region

The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
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integral to plasma membrane

integral to plasma membrane

Penetrating at least one phospholipid bilayer of a plasma membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer.
GO Category: 
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.170000 1.12

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: