Rv3534c 4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.-)


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv3534c hsaF 4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.-) CDS 3972552 3973592 - 1 041 346 FALSE

Rv3534c (4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.-)) is predicted to be co-regulated in modules bicluster_0199 with residual 0.54 and bicluster_0337 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0199 and 0.00 and 0.00 for bicluster_0337 respectively.

These modules are enriched for following go terms: aspartate carbamoyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE 4-HYDROXY-2-OXOVALERATE ALDOLASE [HOA] 4-hydroxy-2-ketovalerate aldolase
Operon # Operon
2309 - -
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Phenylalanine metabolism

Total items in this category:  


Benzoate degradation

Total items in this category:  


Dioxin degradation

Total items in this category:  


Xylene degradation

Total items in this category:  


Ethylbenzene degradation

Total items in this category:  


Metabolic pathways

Total items in this category:  


Microbial metabolism in diverse environments

Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15610670 NP_218051.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.23

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: