Organism : Pseudomonas aeruginosa | Module List :
PA0381 thiG

thiazole synthase (NCBI)

CircVis
Functional Annotations (5)
Function System
Uncharacterized enzyme of thiazole biosynthesis cog/ cog
catalytic activity go/ molecular_function
thiamine biosynthetic process go/ biological_process
Thiamine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA0381
(Mouseover regulator name to see its description)

PA0381 is regulated by 39 influences and regulates 0 modules.
Regulators for PA0381 thiG (39)
Regulator Module Operator
PA0179 272 tf
PA0815 272 tf
PA0890 272 tf
PA0961 272 tf
PA2957 272 tf
PA3604 272 tf
PA4052 272 tf
PA4094 272 tf
PA4238 272 tf
PA4270 272 tf
PA4703 272 tf
PA4745 272 tf
PA4853 272 tf
PA4890 272 tf
PA5261 272 tf
PA5550 272 tf
PA5562 272 tf
PA0207 114 tf
PA0961 114 tf
PA1125 114 tf
PA1201 114 tf
PA1241 114 tf
PA1627 114 tf
PA1713 114 tf
PA1850 114 tf
PA2047 114 tf
PA2100 114 tf
PA2622 114 tf
PA2957 114 tf
PA3027 114 tf
PA3135 114 tf
PA3604 114 tf
PA3622 114 tf
PA3804 114 tf
PA4052 114 tf
PA4451 114 tf
PA4600 114 tf
PA4853 114 tf
PA5342 114 tf

Warning: PA0381 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3060 1.50e+01 gAAA.ccGCGa
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3061 3.00e+03 cTTCCaTc
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3372 9.30e+01 AtaATccCGcgccTT
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3373 4.60e+03 AtTGtg.TTTtTggAG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA0381

PA0381 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Uncharacterized enzyme of thiazole biosynthesis cog/ cog
catalytic activity go/ molecular_function
thiamine biosynthetic process go/ biological_process
Thiamine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Module neighborhood information for PA0381

PA0381 has total of 29 gene neighbors in modules 114, 272
Gene neighbors (29)
Gene Common Name Description Module membership
PA0022 PA0022 hypothetical protein (NCBI) 114, 384
PA0381 thiG thiazole synthase (NCBI) 114, 272
PA0582 folB dihydroneopterin aldolase (NCBI) 114, 181
PA0654 speD S-adenosylmethionine decarboxylase proenzyme (NCBI) 272, 308
PA0775 PA0775 hypothetical protein (NCBI) 114, 135
PA0858 PA0858 hypothetical protein (NCBI) 114, 404
PA0944 purN phosphoribosylglycinamide formyltransferase (NCBI) 272, 520
PA0945 purM phosphoribosylaminoimidazole synthetase (NCBI) 272, 308
PA0961 PA0961 probable cold-shock protein (NCBI) 272, 332
PA1964 PA1964 probable ATP-binding component of ABC transporter (NCBI) 114, 230
PA3000 aroP1 aromatic amino acid transport protein AroP1 (NCBI) 114, 207
PA3019 PA3019 probable ATP-binding component of ABC transporter (NCBI) 39, 114
PA3210 trkH potassium uptake protein TrkH (NCBI) 114, 207
PA3264 PA3264 probable transporter (NCBI) 114, 170
PA3308 hepA ATP-dependent helicase HepA (NCBI) 90, 272
PA3585 glpM membrane protein GlpM (NCBI) 72, 114
PA3675 PA3675 hypothetical protein (NCBI) 114, 143
PA3680 PA3680 hypothetical protein (NCBI) 114, 438
PA3903 prfC peptide chain release factor 3 (NCBI) 90, 272
PA4006 nadD nicotinic acid mononucleotide adenyltransferase (NCBI) 114, 440
PA4671 PA4671 50S ribosomal protein L25 (NCBI) 272, 510
PA4672 PA4672 peptidyl-tRNA hydrolase (NCBI) 272, 510
PA4673 PA4673 hypothetical protein (NCBI) 272, 510
PA4960 PA4960 probable phosphoserine phosphatase (NCBI) 114, 181
PA5129 grx glutaredoxin (NCBI) 272, 332
PA5138 PA5138 hypothetical protein (NCBI) 114, 218
PA5139 PA5139 hypothetical protein (NCBI) 114, 207
PA5192 pckA phosphoenolpyruvate carboxykinase (NCBI) 90, 272
PA5479 gltP proton-glutamate symporter (NCBI) 269, 272
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA0381
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend