Organism : Pseudomonas aeruginosa | Module List :
PA0394

hypothetical protein (NCBI)

CircVis
Functional Annotations (2)
Function System
Predicted enzyme with a TIM-barrel fold cog/ cog
TIGR00044 tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA0394
(Mouseover regulator name to see its description)

PA0394 is regulated by 32 influences and regulates 0 modules.
Regulators for PA0394 (32)
Regulator Module Operator
PA0393 393 tf
PA0424 393 tf
PA0576 393 tf
PA0652 393 tf
PA0905 393 tf
PA1399 393 tf
PA2123 393 tf
PA2206 393 tf
PA2588 393 tf
PA3126 393 tf
PA3225 393 tf
PA4269 393 tf
PA5324 393 tf
PA0393 499 tf
PA0455 499 tf
PA0780 499 tf
PA0890 499 tf
PA1184 499 tf
PA1226 499 tf
PA1859 499 tf
PA2957 499 tf
PA3220 499 tf
PA3321 499 tf
PA3604 499 tf
PA3948 499 tf
PA4451 499 tf
PA4530 499 tf
PA5239 499 tf
PA5308 499 tf
PA5344 499 tf
PA5550 499 tf
PA5562 499 tf

Warning: PA0394 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3610 2.10e-02 TTGAAcaGccG.aaacCGcCCCta
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3611 8.50e+03 GGTTTCCA
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3812 7.30e-16 aag.aAa.gcgGctAgcaTag
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3813 1.60e-04 gcCg.gA..tttAtTCaGAtTtcC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA0394

PA0394 is enriched for 2 functions in 3 categories.
Enrichment Table (2)
Function System
Predicted enzyme with a TIM-barrel fold cog/ cog
TIGR00044 tigr/ tigrfam
Module neighborhood information for PA0394

PA0394 has total of 45 gene neighbors in modules 393, 499
Gene neighbors (45)
Gene Common Name Description Module membership
PA0067 prlC oligopeptidase A (NCBI) 293, 393
PA0392 PA0392 hypothetical protein (NCBI) 18, 499
PA0393 proC pyrroline-5-carboxylate reductase (NCBI) 389, 393
PA0394 PA0394 hypothetical protein (NCBI) 393, 499
PA0653 PA0653 hypothetical protein (NCBI) 212, 499
PA0778 icp inhibitor of cysteine peptidase (NCBI) 315, 499
PA0779 PA0779 probable ATP-dependent protease (NCBI) 393, 509
PA0917 kup potassium uptake protein Kup (NCBI) 3, 499
PA1303 PA1303 probable signal peptidase (NCBI) 20, 499
PA1304 PA1304 probable oligopeptidase (NCBI) 499, 517
PA1306 PA1306 probable HIT family protein (NCBI) 100, 499
PA1596 htpG heat shock protein 90 (NCBI) 393, 509
PA1597 PA1597 hypothetical protein (NCBI) 393, 509
PA1798 PA1798 probable two-component sensor (NCBI) 384, 499
PA1799 PA1799 probable two-component response regulator (NCBI) 384, 499
PA1803 lon Lon protease (NCBI) 393, 464
PA2545 xthA exodeoxyribonuclease III (NCBI) 384, 499
PA2830 htpX heat shock protein HtpX (NCBI) 393, 524
PA3126 ibpA heat-shock protein IbpA (NCBI) 10, 393
PA3338 PA3338 hypothetical protein (NCBI) 384, 499
PA3538 PA3538 probable ATP-binding component of ABC transporter (NCBI) 4, 499
PA3738 xerD tyrosine recombinase (NCBI) 374, 499
PA4061 PA4061 probable thioredoxin (NCBI) 393, 509
PA4385 groEL chaperonin GroEL (NCBI) 393, 509
PA4386 groES co-chaperonin GroES (NCBI) 393, 509
PA4387 PA4387 hypothetical protein (NCBI) 393, 509
PA4542 clpB ClpB protein (NCBI) 393, 509
PA4643 PA4643 hypothetical protein (NCBI) 170, 499
PA4646 upp uracil phosphoribosyltransferase (NCBI) 60, 499
PA4647 uraA uracil permease (NCBI) 60, 499
PA4751 ftsH cell division protein FtsH (NCBI) 393, 488
PA4759 dapB dihydrodipicolinate reductase (NCBI) 393, 509
PA4760 dnaJ DnaJ protein (NCBI) 393, 509
PA4761 dnaK molecular chaperone DnaK (NCBI) 393, 509
PA4762 grpE heat shock protein GrpE (NCBI) 393, 509
PA5053 hslV ATP-dependent protease peptidase subunit (NCBI) 393, 509
PA5054 hslU ATP-dependent protease ATP-binding subunit (NCBI) 393, 509
PA5055 PA5055 hypothetical protein (NCBI) 393, 509
PA5124 ntrB two-component sensor NtrB (NCBI) 203, 499
PA5134 PA5134 probable carboxyl-terminal protease (NCBI) 94, 499
PA5221 PA5221 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (NCBI) 3, 499
PA5222 PA5222 hypothetical protein (NCBI) 353, 499
PA5226 PA5226 hypothetical protein (NCBI) 57, 499
PA5227 PA5227 hypothetical protein (NCBI) 499, 551
PA5241 ppx exopolyphosphatase (NCBI) 123, 499
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA0394
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend